Protein Info for GFF980 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01039: Carboxyl_trans
  • amino acids 48 to 525 (478 residues), 467.9 bits, E=3.8e-144
• PF03255: ACCA
  • amino acids 89 to 210 (122 residues), 31.6 bits, E=1e-11
  • amino acids 341 to 425 (85 residues), 29.6 bits, E=4.2e-11

Best Hits

Swiss-Prot: 54% identical to MCCB_MOUSE: Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (Mccc2) from Mus musculus

KEGG orthology group: None (inferred from 98% identity to pol:Bpro_5271)

MetaCyc: 54% identical to propionyl-CoA carboxylase alpha subunit (Chloroflexus aurantiacus)
Propionyl-CoA carboxylase. [EC: 6.4.1.3]

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

• propanoyl CoA degradation I (3/3 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• L-leucine degradation I (5/6 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• 3-hydroxypropanoate cycle (8/13 steps found)
• methylaspartate cycle (12/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)
• superpathway of the 3-hydroxypropanoate cycle (9/18 steps found)
• mycolate biosynthesis (26/205 steps found)
• superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.3, 6.4.1.4

Use Curated BLAST to search for 6.4.1.3 or 6.4.1.4

Protein Sequence (535 amino acids)

>GFF980 Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTILSSRISTSDDEFRLNQKAYQAVIADLQSRRQLALAGGPPKAREKHLARNKILPRHRV
EVLLDPGSPFLEVGMLAGEGMYDGVPPGASIITGIGLVQGRPCMIIANDATVKGGTYYGM
TCKKHVRAQQIAWAHRLPCITLVDSGGAFLPEMANIFPDVGQFGSIFNNQVRMSAEGIQQ
IAVVMGPCTAGGAYIPALCDEAVIVKEQGYMYLGGPELTFAATGETVDAESLGGAKMHCS
VSGVTDHIAEDDRHALAITREIVRDLGEQPKPRWTRQPSVPPRFDPREIYGIISRDTKIP
TDTREILARFVDDSRFQEFKPMYGDTLLTGFARIHGHEVGILANQGVLFPESAMKAAHFI
DLCCQRDIPLLFMADVTGFMVGRAAEQAGIAKAGAKMITAMASANVPKYTIIMGASYGAG
YLAMCGRPFNPTAMFAWPTGRAAIMGPDQAATVMALVRRQINKTEGKEWTPEEEEAFKAP
VRKIYEDFQPAANFSSNLWVDGIIDPVETRDAMGLLLDLASRTAAKPTPFGVFRF