Protein Info for GFF978 in Variovorax sp. SCN45

Annotation: Propionate--CoA ligase (EC 6.2.1.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 637 transmembrane" amino acids 123 to 145 (23 residues), see Phobius details TIGR02316: propionate--CoA ligase" amino acids 3 to 634 (632 residues), 1093.7 bits, see alignment E=0 PF16177: ACAS_N" amino acids 5 to 58 (54 residues), 81.7 bits, see alignment 3.8e-27 PF00501: AMP-binding" amino acids 60 to 453 (394 residues), 246.4 bits, see alignment E=6.6e-77 PF13193: AMP-binding_C" amino acids 517 to 600 (84 residues), 87.9 bits, see alignment E=1e-28

Best Hits

Swiss-Prot: 65% identical to PRPE_SALTY: Propionate--CoA ligase (prpE) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01908, propionyl-CoA synthetase [EC: 6.2.1.17] (inferred from 94% identity to vap:Vapar_2170)

MetaCyc: 65% identical to propionate-CoA ligase (Salmonella enterica enterica serovar Typhimurium)
Propionate--CoA ligase. [EC: 6.2.1.17]

Predicted SEED Role

"Propionate--CoA ligase (EC 6.2.1.17)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 6.2.1.17)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (637 amino acids)

>GFF978 Propionate--CoA ligase (EC 6.2.1.17) (Variovorax sp. SCN45)
MSRFEEFYRQSVDEPEAFWAEQAKLIDWHTPAQQILDASQPPFARWFVGGTTNLCHNAVD
RHLATRGGQPALVFVSTETGVEKSYSFDELHAEVQRTAASLMELGVGKGDRVLIYMPMIP
EAAFAMLACARIGAIHCVVFGGFASVSLATRIEDAEPKVIVSADAGSRGGKVIAYKPLLD
EAIRLSKHKPSAVLLTDRGLAPMDLAAGRDHLAAELRQRHIDAKVPCTWMESTDISYTIY
TSGTTGKPKGVQRDVGGYAVALAASMKHIFDGRPGETYFSTSDIGWVVGHSYIVYGPLIA
GMATIMYEGLPTQGIDRQPDGGIWWRLVEKYRVTVMFSAPTAVRVLKKQDPALLKKYDLS
TLRALFLAGEPLDEPTARWISEGLGVPIIDNYWQTESGWPMITIANGVEAKASKFGSPGV
PMYGYRIKILHESTGEELTGANEKGVVVVEGPTPPGFMLTVWKDDARFVNTYWKSVPGKM
VYSTFDWGIRDEDGYFYILGRTDDVINVAGHRLGTREIEESISGHANVAEVAVVGVADAL
KGQVAMAFVVPKDGKAVADADLALKLEGEIMKLVADQLGALARPARVRFVSGLPKTRSGK
LLRRAIQAVCEQRDPGDLTTIDDPATLQQIKQLLSSV