Protein Info for GFF978 in Methylophilus sp. DMC18

Annotation: Phosphate import ATP-binding protein PstB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 259 TIGR00972: phosphate ABC transporter, ATP-binding protein" amino acids 11 to 259 (249 residues), 380.9 bits, see alignment E=1.2e-118 PF00005: ABC_tran" amino acids 26 to 184 (159 residues), 107 bits, see alignment E=1.8e-34 PF13304: AAA_21" amino acids 127 to 214 (88 residues), 34 bits, see alignment E=5.2e-12

Best Hits

Swiss-Prot: 90% identical to PSTB_METFK: Phosphate import ATP-binding protein PstB (pstB) from Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)

KEGG orthology group: K02036, phosphate transport system ATP-binding protein [EC: 3.6.3.27] (inferred from 90% identity to mfa:Mfla_0792)

MetaCyc: 55% identical to phosphate ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-27-RXN [EC: 7.3.2.1]; 7.3.2.1 [EC: 7.3.2.1]

Predicted SEED Role

"Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.3.27 or 7.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (259 amino acids)

>GFF978 Phosphate import ATP-binding protein PstB (Methylophilus sp. DMC18)
MATVTTAIKAEARDLNFFYSNGARALKGISMPLYENKITALIGPSGCGKSTFLRCFNRMH
DLYPGNKYQGQIVMHPDNTNVLDSGVDPIEVRMRISMVFQKPNPFPKSIYENVAYGLRVR
GENNKRIMDDKVEEALKGAALWNEVKDRLHDLAFNLSGGQQQRLCIARALATDPEIMLFD
EPTSALDPIATANIEELMVELRSKLTILVVTHNMQQAARVSDYTAYMYLGELIEYDDTDK
IFTNPSRKETEDYITGKFG