Protein Info for GFF964 in Variovorax sp. SCN45

Annotation: Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) / Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 6 to 120 (115 residues), 137 bits, E=3.4e-44
• PF02882: THF_DHG_CYH_C
  • amino acids 124 to 281 (158 residues), 238.3 bits, E=2.5e-75

Best Hits

Swiss-Prot: 93% identical to FOLD_VARPS: Bifunctional protein FolD (folD) from Variovorax paradoxus (strain S110)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 96% identity to vpe:Varpa_3733)

MetaCyc: 59% identical to methenylTHF cyclohydrolase/methyleneTHF dehydrogenase subunit (Moorella thermoacetica)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• folate transformations III (E. coli) (8/9 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• folate transformations II (plants) (9/11 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
• L-histidine degradation III (4/6 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• folate transformations I (8/13 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (2/10 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Protein Sequence (283 amino acids)

>GFF964 Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) / Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) (Variovorax sp. SCN45)
MTAQLIDGNALAQTIRAEVAGRTAALKARGVNPALSIILVGEDPASQVYTKHKVNDSAQT
GLSATLETYPADMTEADLLARIRALNDDPKVHGILVQLPLPKHMDSQKVIETISPSKDVD
GFHVASAGALMTGAPGFWPCTPHGCMKMLESIGYNLRGKHAVVIGRSNIVGKPMAMMLLA
KSATVTICHSATQDLGAITRQADVIVAAVGKRNLLTADMVKPGAVVIDVGMNRKEDGKLA
GDVDFDGVKEVASWITPVPGGVGPMTRAMLLANTLEAAERAAK