Protein Info for GFF963 in Pseudomonas sp. DMC3

Annotation: UDP-glucose 6-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03721: UDPG_MGDP_dh_N
  • amino acids 1 to 188 (188 residues), 203.5 bits, E=5.9e-64
• TIGR03026: nucleotide sugar dehydrogenase
  • amino acids 1 to 421 (421 residues), 448.8 bits, E=8.4e-139
• PF00984: UDPG_MGDP_dh
  • amino acids 205 to 297 (93 residues), 129 bits, E=1.5e-41
• PF03720: UDPG_MGDP_dh_C
  • amino acids 321 to 425 (105 residues), 107.2 bits, E=1.3e-34

Best Hits

KEGG orthology group: K00012, UDPglucose 6-dehydrogenase [EC: 1.1.1.22] (inferred from 94% identity to pfo:Pfl01_2024)

Predicted SEED Role

"UDP-glucose dehydrogenase (EC 1.1.1.22)" in subsystem Lipid A-Ara4N pathway ( Polymyxin resistance ) or Teichuronic acid biosynthesis (EC 1.1.1.22)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (9/11 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (5/6 steps found)
• UDP-α-D-glucuronate biosynthesis (from UDP-glucose) (1/1 steps found)
• UDP-α-D-xylose biosynthesis (1/2 steps found)
• UDP-sugars interconversion (2/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)

KEGG Metabolic Maps

• Ascorbate and aldarate metabolism
• Nucleotide sugars metabolism
• Pentose and glucuronate interconversions
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.22

Use Curated BLAST to search for 1.1.1.22

Protein Sequence (450 amino acids)

>GFF963 UDP-glucose 6-dehydrogenase (Pseudomonas sp. DMC3)
MDVSVFGTGYVGLIQAAALADVGHRVLCIDIDPNKIKQLQQAVPPISEPGLSATLEENIK
AGRLLFSTQASDAVAHAELIFIAVGTPADEDGSADLSHVLNVARQIAGLMEADRTLIIKS
TVPVGTADQVLATATSELLLRGKTELQVRVVSNPEFLKEGSALADCMRPDRIIIGTRDDA
AREQMSELYAPFCRNHEKLMFMDNRSAELTKYAANAMLATRISFMNELANLTELLGADIE
AVRKGIGSDPRIGYHFIYPGCGFGGSCFPKDLRALLHTAEHNGMPLKLLRSVTDVNDSQR
HILFSKLRAQFPQGLAGKSIAVWGLAFKPNTDDMREAPSRYLMDALWAEGASVQAYDPEA
MSECRRIYGYRNDLHLCATRDDTLEDADALVICTEWKNFRVVDFELLAEKLRSKVIIDGR
NLYNPEQVAAAGLHYSGIGLRHIAPEGLRP