Protein Info for GFF960 in Xanthobacter sp. DMC5

Annotation: Glutamate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00749: tRNA-synt_1c
  • amino acids 3 to 306 (304 residues), 323.5 bits, E=1.1e-100
• TIGR00464: glutamate--tRNA ligase
  • amino acids 4 to 470 (467 residues), 513.9 bits, E=2.2e-158
• PF19269: Anticodon_2
  • amino acids 335 to 468 (134 residues), 117.1 bits, E=8.7e-38

Best Hits

Swiss-Prot: 91% identical to SYE1_XANP2: Glutamate--tRNA ligase 1 (gltX1) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 91% identity to xau:Xaut_4369)

Predicted SEED Role

"Glutamyl-tRNA synthetase (EC 6.1.1.17)" in subsystem Heme and Siroheme Biosynthesis or tRNA aminoacylation, Glu and Gln (EC 6.1.1.17)

MetaCyc Pathways

• tRNA charging (19/21 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (26/33 steps found)
• superpathway of heme b biosynthesis from glutamate (7/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (4/6 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (24/36 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Glutamate metabolism
• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.17

Use Curated BLAST to search for 6.1.1.17

Protein Sequence (471 amino acids)

>GFF960 Glutamate--tRNA ligase (Xanthobacter sp. DMC5)
MSQIVTRFAPSPTGFLHIGGARTALFNWLYARHTGGKMLLRIEDTDRQRSTKEAIDAILE
GLSWLGIDWDGDPIYQFARAERHQAAVAEMLAKGNAYRCYATPQELDEMRELARKEGRPP
RYDGRWRDRDPSEAPADRAPVIRLRAPQEGETVIDDMVQGTVTFPNKDLDDLVLLRSDGT
PTYMLAVVVDDHDMGVTHIIRGDDHLTNAARQTQIYRALGWDVPRMAHIPLIHGPDGAKL
SKRHGALGVDAYRDMGYLPAALRNYLVRLGWSHGDQEVFSTDEMVEFFDLDKVGRSAARF
DFAKLESLNGHYMRASSDADLLAAIDALVPYLPDAEHRLPRMTDTRRAQLAAAMPGLKER
AKTLVELLDNAEFIFVERPIPLDEKATTLLSAEGRAHLGRLVPLLEAVEWTAAATEEVVR
RYAEAQSVKLGAVAQPLRAALTGKSTSPPVFDVLIVLGREESLGRLRDQAI