Protein Info for GFF960 in Variovorax sp. SCN45

Annotation: Dihydroorotate dehydrogenase (quinone) (EC 1.3.5.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01036: dihydroorotate dehydrogenase (fumarate)
  • amino acids 10 to 352 (343 residues), 389.7 bits, E=5.1e-121
• PF01180: DHO_dh
  • amino acids 55 to 352 (298 residues), 266 bits, E=1.9e-83

Best Hits

Swiss-Prot: 79% identical to PYRD_POLSJ: Dihydroorotate dehydrogenase (quinone) (pyrD) from Polaromonas sp. (strain JS666 / ATCC BAA-500)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 92% identity to vpe:Varpa_3737)

Predicted SEED Role

"Dihydroorotate dehydrogenase (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

• UMP biosynthesis II (6/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.3.3.1, 1.3.98.1

Use Curated BLAST to search for 1.3.3.1 or 1.3.98.1

Protein Sequence (359 amino acids)

>GFF960 Dihydroorotate dehydrogenase (quinone) (EC 1.3.5.2) (Variovorax sp. SCN45)
MPLLMPSSLYGLARPFLFGFDPEHAHELTLDGLARTQNTPLACAYSAPRVSDPIQLAGLS
FPNRVGLAAGLDKNARCIDAFAAMGFGFVEVGTVTPKGQPGNPKPRMFRLPQRDALINRL
GFNNEGLEAFLANVQKARFRKAGGAAKQPMLLGLNIGKNAATPIERAVDDYLACLDGVYP
HADYVTINISSPNTANLRSLQSDEALDALLGAVAERREALATRHAKRSPLFVKIAPDLDE
TQVAVIAATLKRHGMDGVIATNTTLARDAVKGLPHAEEAGGLSGAPVREASNRVIAQLRA
ALGSGFPIIGVGGILSGADAKAKIAAGADVVQIYTGLIYRGPALVREAAQALLHDRSKA