Protein Info for GFF958 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: Naphthoate synthase (EC 4.1.3.36)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 54% identical to MENB_STAHJ: 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB) from Staphylococcus haemolyticus (strain JCSC1435)
KEGG orthology group: K07536, 2-ketocyclohexanecarboxyl-CoA hydrolase [EC: 3.1.2.-] (inferred from 73% identity to reu:Reut_B3916)MetaCyc: 70% identical to BadI (Rhodopseudomonas palustris)
3.7.1.-
Predicted SEED Role
"Naphthoate synthase (EC 4.1.3.36)" in subsystem Menaquinone and Phylloquinone Biosynthesis (EC 4.1.3.36)
MetaCyc Pathways
- superpathway of chorismate metabolism (43/59 steps found)
- benzoyl-CoA degradation III (anaerobic) (5/9 steps found)
- 2-carboxy-1,4-naphthoquinol biosynthesis (3/7 steps found)
- superpathway of menaquinol-8 biosynthesis I (5/10 steps found)
- superpathway of demethylmenaquinol-8 biosynthesis I (4/9 steps found)
- superpathway of menaquinol-10 biosynthesis (4/10 steps found)
- superpathway of menaquinol-11 biosynthesis (4/10 steps found)
- superpathway of menaquinol-12 biosynthesis (4/10 steps found)
- superpathway of menaquinol-13 biosynthesis (4/10 steps found)
- superpathway of menaquinol-6 biosynthesis (4/10 steps found)
- superpathway of menaquinol-7 biosynthesis (4/10 steps found)
- superpathway of menaquinol-9 biosynthesis (4/10 steps found)
- superpathway of demethylmenaquinol-6 biosynthesis I (3/9 steps found)
- superpathway of demethylmenaquinol-9 biosynthesis (3/9 steps found)
- superpathway of phylloquinol biosynthesis (4/15 steps found)
KEGG Metabolic Maps
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Biosynthesis of unsaturated fatty acids
- Fatty acid biosynthesis
- Limonene and pinene degradation
- Ubiquinone and menaquinone biosynthesis
- alpha-Linolenic acid metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.1.2.-, 4.1.3.36
Use Curated BLAST to search for 3.1.2.- or 4.1.3.36
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (263 amino acids)
>GFF958 Naphthoate synthase (EC 4.1.3.36) (Hydrogenophaga sp. GW460-11-11-14-LB1) MEFKDILYEELDSVAYIAINRPEVMNAFRGQTVEELLQAFMKAGWDKRIAAIVLTGTGDR AFCTGGDQSAHAGQYDGRGTIGIPVEEFHSAIRDVPKPVIARVQGYAIGGGNVLATLCDM TIASEKAVFGQVGPKVGSVDPGWGTAYLSHVVGEKKAREIWYMCRRYSAQEALAMGLCNT VVPHDQLDAEVARWCKEIVEKSPTALAIAKRSFNASTEHIRGIGSLGLQALSLYYDTAES KEGVSAFLEKRRPDFRQWANGRK