Protein Info for GFF947 in Methylophilus sp. DMC18

Annotation: NADH-quinone oxidoreductase subunit H

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00146: NADHdh
  • amino acids 23 to 329 (307 residues), 420.5 bits, E=2e-130

Best Hits

Swiss-Prot: 84% identical to NUOH_METFK: NADH-quinone oxidoreductase subunit H (nuoH) from Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)

KEGG orthology group: K00337, NADH dehydrogenase I subunit H [EC: 1.6.5.3] (inferred from 91% identity to meh:M301_1764)

MetaCyc: 55% identical to MbhM (Pyrococcus furiosus)
Ferredoxin hydrogenase. [EC: 1.12.7.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain H (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
• hydrogen production III (1/1 steps found)
• hydrogen production VIII (1/1 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• NADH to cytochrome bd oxidase electron transfer I (1/2 steps found)
• hydrogen production VI (1/2 steps found)
• superpathway of hydrogen production (1/2 steps found)
• superpathway of photosynthetic hydrogen production (3/6 steps found)
• Fe(II) oxidation (2/6 steps found)
• NAD(P)/NADPH interconversion (2/6 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (3/9 steps found)
• L-glutamate degradation VII (to butanoate) (1/12 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism
• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.12.7.2 or 1.6.5.3

Protein Sequence (342 amino acids)

>GFF947 NADH-quinone oxidoreductase subunit H (Methylophilus sp. DMC18)
MHFFAELFGSYWTDVQLVVWTLIKIVAIVLPLMIGVAYLTLAERKVIGYMQVRIGPNRVG
YWGLLQPLADGLKLLFKEIILPTASNKALFFIGPVLAIAPAFAAWAVVPFDATLVLANVD
AGLLYILAMTSVAVYGVIIAGWASNSKYAFLGSLRSAAQIVSYEIAMGFTLVGVLMCANS
LNLGKIVMGQEGGFWHWYFLPLFPLFVVYFISAVAETNRAPFDVAEGESEIVAGFHVEYS
GMAFAVFFLAEYANMMLVSMLAALMFLGGWLSPVPFLPDSIVWLLAKVAFLLFLFLWFRA
TFPRYRYDQIMRLGWKVFIPITLVWIIVVGGMMQTQWAYLFH