Protein Info for PS417_04800 in Pseudomonas simiae WCS417

Annotation: alginate O-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 394 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF16822: ALGX" amino acids 81 to 345 (265 residues), 300.9 bits, see alignment E=5.1e-94

Best Hits

Swiss-Prot: 98% identical to ALGJ_PSEFL: Probable alginate O-acetylase AlgJ (algJ) from Pseudomonas fluorescens

KEGG orthology group: None (inferred from 98% identity to pfs:PFLU0981)

MetaCyc: 54% identical to AlgJ (Pseudomonas aeruginosa)
RXN-16462

Predicted SEED Role

"alginate biosynthesis protein AlgJ" in subsystem Alginate metabolism

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1UPK7 at UniProt or InterPro

Protein Sequence (394 amino acids)

>PS417_04800 alginate O-acetyltransferase (Pseudomonas simiae WCS417)
MTRSLRVLYIGLFLVLLLALGAWSLRSFFGFSTNADATVLNGRWTKAVETHYDDEFPIKR
IGTNLWAALDYKLFNEGRPGVVLGRDHWLYSDEEFNPAVNEDQNLEGNYALVEGVRQKLK
AQGIQLVMAIVPAKVRLYPEHLGEVKPASIHANLYQDFHARVAADKIIAPDLLGPLQQAK
LGGKQVFLRTDTHWTPDGAEIAAKQLAKTIADKTPLHGEPQRFITEAEKTEPHKGDLRLF
LPLDPLFENLMPPKEPLEKRVTHLAETQGDDALFSDSETPVALVGTSYSANPNWNFVGAL
KQALGSDVVSYAEDGHGPILPMLSYLKSDDFKNSPPQVLIWEFPERYLPVNNEIGDADPS
WVAQLKQAGSRQQNMALNTPVKNQKSETPDRAQN