Protein Info for GFF946 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 46% identical to PYCA_METJA: Pyruvate carboxylase subunit A (pycA) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
KEGG orthology group: None (inferred from 52% identity to rbi:RB2501_01580)MetaCyc: 44% identical to pyruvate carboxylase subunit A (Methanothermobacter thermautotrophicus Delta H)
Pyruvate carboxylase. [EC: 6.4.1.1]
Predicted SEED Role
"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)
MetaCyc Pathways
- gluconeogenesis III (11/12 steps found)
- L-leucine degradation I (5/6 steps found)
- reductive TCA cycle I (7/11 steps found)
- incomplete reductive TCA cycle (4/7 steps found)
- (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
- gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Citrate cycle (TCA cycle)
- Pyruvate metabolism
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 6.4.1.4
Use Curated BLAST to search for 6.4.1.1 or 6.4.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (489 amino acids)
>GFF946 Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) (Hydrogenophaga sp. GW460-11-11-14-LB1) VFQKVLIANRGEIALRILHTLKGMGLATVVAHHAVDADSPAVLEADESVELHGLTPVAAY LDIAQLVEACRRIGAQALHPGYGFLSENAAFARALEAAGIVFIGPRPDSIELMGDKVRAR SFVAQRGFPVAPSAIEDDDPASFVERARALGAPLLIKPSAGGGGKGMRIVRDLAVLDREI ETARREGERYFGDGRLFVERYVERPRHIEVQVMGDAHGQLVHFWERECSVQRRFQKIIEE TPSPALSPVQRHDICEAAAGIARAANYRGAGTVEFIYGAGGEFYFLEMNTRLQVEHPVTE MVTGFDLVREQVRVAQGEPLGYTQADIVQLGHAIECRLYAEDAEHDFRPSTGDILCWRPP SGSGVRVDAGVREGQAVTAAFDPMIAKIICHGNTRAQAIERSILALEQSVLLGLTTNARY LTRVLRHAEFRAGQADTGMLARCADELRESLTPDDIDLVLASAVLADRELLRAVHSIPAM HAAMGPWRN