Protein Info for PS417_04790 in Pseudomonas simiae WCS417

Annotation: mannose-1-phosphate guanylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
  • amino acids 1 to 468 (468 residues), 725.8 bits, E=1.1e-222
• PF00483: NTP_transferase
  • amino acids 3 to 284 (282 residues), 220.2 bits, E=6.8e-69
• PF12804: NTP_transf_3
  • amino acids 4 to 129 (126 residues), 30.5 bits, E=8e-11
• PF01050: MannoseP_isomer
  • amino acids 314 to 464 (151 residues), 257.6 bits, E=7.2e-81
• PF07883: Cupin_2
  • amino acids 381 to 448 (68 residues), 41.7 bits, E=1.6e-14

Best Hits

Swiss-Prot: 98% identical to ALGA_PSEFL: Alginate biosynthesis protein AlgA (algA) from Pseudomonas fluorescens

KEGG orthology group: None (inferred from 98% identity to pfs:PFLU0979)

MetaCyc: 80% identical to phosphomannose isomerase / GDP-D-mannose pyrophosphorylase (Pseudomonas aeruginosa)
Mannose-6-phosphate isomerase. [EC: 5.3.1.8]; Mannose-1-phosphate guanylyltransferase. [EC: 5.3.1.8, 2.7.7.13]

Predicted SEED Role

"Mannose-6-phosphate isomerase (EC 5.3.1.8) / Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22, EC 5.3.1.8)

MetaCyc Pathways

• GDP-mannose biosynthesis (4/4 steps found)
• colanic acid building blocks biosynthesis (8/11 steps found)
• β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
• D-mannose degradation I (1/2 steps found)
• D-mannose degradation II (1/2 steps found)
• mannitol degradation II (2/4 steps found)
• mannitol biosynthesis (1/3 steps found)
• 1,5-anhydrofructose degradation (2/5 steps found)
• β-(1,4)-mannan degradation (2/7 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (6/14 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.13, 2.7.7.22, 5.3.1.8

Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22 or 5.3.1.8

Sequence Analysis Tools

See A0A1N7U504 at UniProt or InterPro

Protein Sequence (483 amino acids)

>PS417_04790 mannose-1-phosphate guanylyltransferase (Pseudomonas simiae WCS417)
MIPVILSGGSGSRLWPLSRKQFPKQFLALTGEHTLFQQTLERLVFEGMDSPIVVCNKDHR
FIVNEQLAARKLESQRILMEPFGRNTAPAVALTAMMLVNEGRDELMLVLPADHVIDDQKA
LQRALALATVAAERGEMVLFGVPATRPETGYGYIKSTNDSLLPEGVSRVEQFVEKPNEKR
AMEFVKSGGYFWNSGMFLFRASRFLEELKKHDPDIYDTCLLTLERSEQTADTVSFDEATF
ACCPDNSIDYAVMEKTQRACVVPLSAGWSDVGCWASLWAVNDKDVHGNVSKGDVVIQDSR
NCMIHGNGKLVSVIGLDNIVVVETKDAMMIAHKDKVQGVKQMVSTLNDQGRSETQNHCEV
YRPWGSYDSVDMGGRFQVKHISVKPGACLSLQMHHHRAEHWIVVSGTAEVTCDENVFLLT
ENQSTYIPIASVHRLRNPGKIPLEIIEVQSGSYLGEDDIERFEDIYGRSTPVERGVSVKT
IAQ