Protein Info for GFF944 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 50% identical to MCCB_CAEEL: Probable methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (F02A9.10) from Caenorhabditis elegans

KEGG orthology group: None (inferred from 56% identity to cti:RALTA_A2850)

MetaCyc: 52% identical to propionyl-CoA carboxylase alpha subunit (Chloroflexus aurantiacus)
Propionyl-CoA carboxylase. [EC: 6.4.1.3]

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

propanoyl CoA degradation I (3/3 steps found)
superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
L-leucine degradation I (5/6 steps found)
2-oxobutanoate degradation I (3/4 steps found)
anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
3-hydroxypropanoate cycle (8/13 steps found)
methylaspartate cycle (12/19 steps found)
3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
superpathway of L-methionine salvage and degradation (8/16 steps found)
superpathway of the 3-hydroxypropanoate cycle (9/18 steps found)
mycolate biosynthesis (26/205 steps found)
superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.3, 6.4.1.4

Use Curated BLAST to search for 6.4.1.3 or 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (532 amino acids)

>GFF944 Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKRIDSRIDTASEAFRRNHQAMSAIVANFRERMEITRHQRPPHEIKRVRAQGKLLVRERL
DLLLDPGTPFLEFSALAACEDYNGEVPGANVVTGLGVVSGREVLIHADDSSVKGGVWYTL
TPRKLARLLDIALENQLPVLHLCDSGGAFLEELSGVYIEGGRVFRNQCLLSKAGVPQVAM
VFGNCTAGGAYIPALCDYSIIVRGTGGVFLGGPPLVKAATGEEHTADEIGGCDLHTQVSG
TVDYPADNEAHAIAICREIVAQFKPATKSYLPERDAEPPYYDPDEMLGIVSPDHRVQFDQ
RELIARIVDGSRFHEYQPAYGTTLICGYAYIWGYRVGILANNGVLFSDSSKKAAHFISLC
NQNRTPLVFLQNTTGYMIGRNYEMEGITKDGAKMLMAQAGSEVPKFTVITSAAFGAGYYG
MCGRAWDPRMIWAWPNVHMGVMGPDQAANTLADVKIAQLRRKGHTPDEAEMAQLRQRVYE
KAERESNAYFATSRLWDDGLLAPTDTRNALGMALSAAAHAPIADPHYGIFRF