Protein Info for GFF937 in Variovorax sp. SCN45

Annotation: Biotin synthase (EC 2.8.1.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00433: biotin synthase
  • amino acids 35 to 330 (296 residues), 392.9 bits, E=4.6e-122
• PF04055: Radical_SAM
  • amino acids 69 to 221 (153 residues), 82.5 bits, E=4.1e-27
• PF06968: BATS
  • amino acids 240 to 331 (92 residues), 92.7 bits, E=1.3e-30

Best Hits

Swiss-Prot: 83% identical to BIOB_ACIAC: Biotin synthase (bioB) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K01012, biotin synthetase [EC: 2.8.1.6] (inferred from 94% identity to vap:Vapar_2132)

MetaCyc: 62% identical to biotin synthase (Escherichia coli K-12 substr. MG1655)
Biotin synthase. [EC: 2.8.1.6]

Predicted SEED Role

"Biotin synthase (EC 2.8.1.6)" in subsystem Biotin biosynthesis (EC 2.8.1.6)

MetaCyc Pathways

• biotin biosynthesis I (14/15 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate I (4/4 steps found)
• biotin biosynthesis II (5/6 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate II (3/4 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate III (3/5 steps found)

KEGG Metabolic Maps

• Biotin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.6

Protein Sequence (344 amino acids)

>GFF937 Biotin synthase (EC 2.8.1.6) (Variovorax sp. SCN45)
MGANDLQQAITLHRPAKAAVPKATDRWSVEAIQALLDKPLMDLLFEAQTVHRQHFPAGDI
ELATLLSVKTGGCPENCGYCPQSAEFDTGVKAQKLMEVEEVVRAAQAAKDAGATRFCMGA
AWRAPKDRDVEKVAVLVEAVKGLGMQTCATLGMLEPHHAHQLKAAGLDYYNHNLDTAPEY
YTDVVDTRTYQERLDTLANVRSAGISVCCGGIVGMGEQPVHRAGLIAQLANLNPYPESVP
INSLVRVPGTPLADSDPVDPFDFVRMIAVARITMPTARVRLSAGRQQLGDAVQALCFMAG
ANSIFYGDKLLVTGNPDVEADTVLLRKLGLNARQVEEQPEGCAA