Protein Info for GFF936 in Variovorax sp. SCN45

Annotation: Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 1 to 108 (108 residues), 154.7 bits, E=4.5e-49
• PF02786: CPSase_L_D2
  • amino acids 115 to 322 (208 residues), 271.6 bits, E=1.7e-84
• PF02222: ATP-grasp
  • amino acids 137 to 292 (156 residues), 28.9 bits, E=3.2e-10
• PF07478: Dala_Dala_lig_C
  • amino acids 141 to 291 (151 residues), 35.9 bits, E=2.3e-12
• PF02785: Biotin_carb_C
  • amino acids 336 to 452 (117 residues), 131 bits, E=7.6e-42
• PF18140: PCC_BT
  • amino acids 472 to 607 (136 residues), 89.3 bits, E=9e-29
• PF13533: Biotin_lipoyl_2
  • amino acids 616 to 650 (35 residues), 27.1 bits, see alignment (E = 1.2e-09)
• PF00364: Biotin_lipoyl
  • amino acids 617 to 680 (64 residues), 60.2 bits, E=5.6e-20

Best Hits

KEGG orthology group: K01965, propionyl-CoA carboxylase alpha chain [EC: 6.4.1.3] (inferred from 97% identity to vpe:Varpa_3760)

Predicted SEED Role

"Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3)" in subsystem Propionyl-CoA to Succinyl-CoA Module (EC 6.4.1.3)

MetaCyc Pathways

• propanoyl CoA degradation I (3/3 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• 3-hydroxypropanoate cycle (9/13 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• methylaspartate cycle (12/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (11/18 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)
• mycolate biosynthesis (26/205 steps found)
• superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.3

Use Curated BLAST to search for 6.4.1.3

Protein Sequence (682 amino acids)

>GFF936 Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3) (Variovorax sp. SCN45)
MFKKILIANRGEIACRVIQTAKKMGILTVAVYSDADKEARHVELADEAVHIGAAPSRESY
LQADRIIEACKKTGAEAVHPGYGFLSENEAFARKVEEEGIAFIGPKHYSIAAMGDKIASK
KLANEAKVNTIPGWNDAIETAERAVEIANDIGYPVMIKASAGGGGKGLRVAFNDKEAFEG
FTSCRNEARNSFGDDRVFIEKFVEEPRHIEIQVLGDSHGNVIYLNERECSIQRRHQKVIE
EAPSPFISDATRKAMGEQAVQLAKAVKYQSAGTVEFVVGKDQSFYFLEMNTRLQVEHPVT
ECITGLDLVELMIRVAAGEKLPLTQAEVKRDGWAIECRINAEDPFRNFLPSTGRLVKFQP
PTETMFASDTAHLNGVRVDTGVYDGGEIPMYYDSMIAKLIVHGKDRQHAIARMREALNGF
VIRGISSNIPFQAALLAHPKFVAGDFNTGFIAEHYGKGFHAEDVPHADPSFLIALAAYVH
RRYRARASGISGQLEGHGVKVGEQFVVVELGPEGKHVHHPVSVTDFQGKTGASAVAVGGK
SYKIDSSLALGAIRVQGIVNDKPFTAQVERGAGKNPLALRVSHDGTQLEAMVLSPLGARL
LELMPYKAPPDLSKFLMSPMPGLLVEVSVQPGQQVQAGEKLAVIEAMKMENVLFATQDGV
VGKISANKGESLAVDQIIMEFN