Protein Info for GFF931 in Variovorax sp. SCN45

Annotation: Methylmalonyl-CoA mutase (EC 5.4.99.2) / B12 binding domain of Methylmalonyl-CoA mutase (EC 5.4.99.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 35 to 556 (522 residues), 891.5 bits, E=1.4e-272
• PF01642: MM_CoA_mutase
  • amino acids 37 to 549 (513 residues), 794.7 bits, E=3.4e-243
• TIGR00640: methylmalonyl-CoA mutase C-terminal domain
  • amino acids 588 to 716 (129 residues), 199.2 bits, E=2.2e-63
• PF02310: B12-binding
  • amino acids 591 to 701 (111 residues), 72.6 bits, E=2.6e-24

Best Hits

Swiss-Prot: 65% identical to MUTA_MOUSE: Methylmalonyl-CoA mutase, mitochondrial (Mmut) from Mus musculus

KEGG orthology group: K01847, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 97% identity to vap:Vapar_2127)

MetaCyc: 70% identical to methylmalonyl-CoA mutase (Cereibacter sphaeroides)
Methylmalonyl-CoA mutase. [EC: 5.4.99.2]

Predicted SEED Role

"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• propanoyl CoA degradation I (3/3 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• 3-hydroxypropanoate cycle (9/13 steps found)
• pyruvate fermentation to propanoate I (4/7 steps found)
• conversion of succinate to propanoate (1/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• methylaspartate cycle (12/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (11/18 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)
• L-glutamate degradation VIII (to propanoate) (4/11 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.2

Protein Sequence (720 amino acids)

>GFF931 Methylmalonyl-CoA mutase (EC 5.4.99.2) / B12 binding domain of Methylmalonyl-CoA mutase (EC 5.4.99.2) (Variovorax sp. SCN45)
MSSTPEPSFKPADLEAWAKAAAKSAPGGDVSALNWITPDGISVKPLYTAADLQGLKYTDT
LPGFEPYLRGPQATMYAVRPWTIRQYAGFSTAEESNAFYRKALAAGGQGVSVAFDLATHR
GYDSDHPRVTGDVGKAGVAIDSVEDMKILFDQIPLDKVSVSMTMNGAVLPVLAGYVVAAE
EQGVAQDQLSGTIQNDILKEFMVRNTYIFPPAPSMRIIGDIIEYTAQKMPKFNSISISGY
HMQEAGANQALELAFTLADGKEYVKTALAKGLDVDGFAGRLSFFWAIGMNFYLEVAKMRA
ARLLWCRIMKEFEPKNPKSLMLRTHCQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSL
HTNALDEAIALPTEFSARIARNTQLIIQEETHITNVIDPWAGSYMMEKLTQDMADAAWAI
IEEVEAMGGMTKAVDSGWAKLKIEAAAAEKQARIDSGKDVIVGVNKYKLKSEDAIDSLSI
DNVKVREQQVARLQSIRASRDGAKVQAALDALTEAAENNTGNLLALSIDAVRLRATVGEI
SDALEKSFGRHRADTQKVTGVYAAAYDSAEGWETLKTEINAFAEEQGRRPRVMISKLGQD
GHDRGAKVVATAFADLGFDVDMGPLFQTPEECARQAIENDVHAVGVSTLAAGHKTLVPAI
IDELRKQGADDIIVFVGGVIPRQDYDFLYEAGVKGIYGPGTPIPASAKDVLEQIRAAVTA