Protein Info for GFF917 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 667 PF00289: Biotin_carb_N" amino acids 1 to 110 (110 residues), 136.9 bits, see alignment E=1.4e-43 PF02786: CPSase_L_D2" amino acids 115 to 321 (207 residues), 212.1 bits, see alignment E=2.3e-66 PF02222: ATP-grasp" amino acids 140 to 291 (152 residues), 36.4 bits, see alignment E=1.6e-12 PF07478: Dala_Dala_lig_C" amino acids 142 to 290 (149 residues), 32.3 bits, see alignment E=2.6e-11 PF02655: ATP-grasp_3" amino acids 151 to 293 (143 residues), 28.9 bits, see alignment E=4e-10 PF02785: Biotin_carb_C" amino acids 335 to 441 (107 residues), 117.1 bits, see alignment E=1.4e-37 PF00364: Biotin_lipoyl" amino acids 594 to 659 (66 residues), 59.4 bits, see alignment E=8.6e-20

Best Hits

KEGG orthology group: None (inferred from 58% identity to pol:Bpro_5270)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (667 amino acids)

>GFF917 Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MFKKILIANRGEIACRIARTCRRLGIAVAGVHSSADRDALHVDLIGESIEIGGAAPAESY
LRIDAVIDAARRTGSQAIHPGFGFLAENAVFARAVEAAGLVFVGPTPEVIERLGDKASAK
REARAAGVAVLGGSELPSRDAAEVAATVRGLPLPVILKAAAGGGGKGMRVVQQLDGLMET
IEAAMREAGSSFGDTALIVEAFVERGRHIEVQIVGDGHGEVIHLFERECTLQRRHQKVIE
EAPAVNLAPALRAAMLGDAVRLGQRLRYRGVGTVEFIVQGDNHHFLEVNPRLQVEHPVTE
EVTGLDVVEIMLRIAAGEGLPLRQSEVQVRGHAIEARVCAEDSEQNFLPSTGELIAVRFP
TSGVRVESGVRAGMAVTPYYDSMLAKLIAHAPTREAALDRLSGALGETQVHGVETNCAFL
QRLLALPATRDASFHTRLIDEVLAEGSQGASSAPLERLAAAAAWWLLDARCNGNQFGAWS
ALDVTGWQMHAGDDRLTPVPALLLRSGGTTHEARFGAVSPESEVTVQIGTERVRLALTAI
AGGNWRLRMGDRHEVLTIVRSVDTVFVQSGGTAHAIGVTSYLNQAITGRQTSDQLRTPMM
GMILKIHVAVGERVRSGDVVATMESMKMELRINADHDGVVRSLSVQNGQMVERGLVVAVV
ESEGTAE