Protein Info for GFF898 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Epi-inositol hydrolase (EC 3.7.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 639 TIGR04377: 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase" amino acids 1 to 637 (637 residues), 942.2 bits, see alignment E=7.5e-288 PF02776: TPP_enzyme_N" amino acids 2 to 127 (126 residues), 60 bits, see alignment E=2.9e-20 PF00205: TPP_enzyme_M" amino acids 213 to 346 (134 residues), 119.6 bits, see alignment E=1.3e-38 PF02775: TPP_enzyme_C" amino acids 435 to 594 (160 residues), 133.7 bits, see alignment E=7.4e-43

Best Hits

Swiss-Prot: 62% identical to IOLD_GEOKA: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (iolD) from Geobacillus kaustophilus (strain HTA426)

KEGG orthology group: K03336, 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase [EC: 3.7.1.-] (inferred from 100% identity to sea:SeAg_B4714)

MetaCyc: 55% identical to 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (Bacillus subtilis subtilis 168)
RXN-14149 [EC: 3.7.1.22]

Predicted SEED Role

"Epi-inositol hydrolase (EC 3.7.1.-)" in subsystem Inositol catabolism (EC 3.7.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.7.1.- or 3.7.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (639 amino acids)

>GFF898 Epi-inositol hydrolase (EC 3.7.1.-) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MAQALVRFLDNQYIDVDGSEIKFVKGIFAIFGHGNVVGLGQALEEDCGQLSVHQGRNEQG
MAHIATGFARQMRRHQIYACTSSVGPGAANMITAAATATANRIPLLLLPGDVYASRQPDP
VLQQVEQEHDLTLSTNDAFRAVSRYWDRITRPEQLMSACISAMRVLTDPADTGAVTLCLP
QDVQGEAWDYPDYFFARRVYRLERHAPTEPMLNEAVALIRRHQRPLIVCGGGVKYSQAEE
ALLRFAERCHLPIAETQAGKGALSSAHPLNVGGIGETGSLAANLLAQEADLIIGVGTRYT
DFTTSSKWIFQNPDVRYLNINVSRFDVFKLDGVQMQGDARVALTQLSERLAQEHYASQWG
ETIHRVRSQYMAEVERVYAVEYSGEGFKPEIEDHMDTQKVFEEFNEITRSWLTQTRVLGV
LNRMLPENALVVAAAGSLPGDLQRVWQSRGENDYHVEYGYSCMGYEVNAALGAKLAQPER
EVYSFVGDGSFMMLHSELVTSVQMGKKITVILLDNMTNGCINNLQMEHGMDSYFTEFRFH
QQESGRQEGGFIPVDFARIAEGYGCKSYRVTTIEQLHEALEDARKQTVSTLIDIKVLPKT
MVHKYLSWWRVGGAQVSRSERIQAVARMLEEHIGQARQY