Protein Info for GFF893 in Methylophilus sp. DMC18

Annotation: 50S ribosomal protein L3 glutamine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 TIGR03533: protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific" amino acids 5 to 289 (285 residues), 422.8 bits, see alignment E=6.5e-131 TIGR00536: methyltransferase, HemK family" amino acids 7 to 291 (285 residues), 237.3 bits, see alignment E=1.8e-74 PF05175: MTS" amino acids 128 to 206 (79 residues), 59.1 bits, see alignment E=1.7e-19 PF06325: PrmA" amino acids 128 to 201 (74 residues), 23.9 bits, see alignment E=1.1e-08 PF09445: Methyltransf_15" amino acids 131 to 203 (73 residues), 25.7 bits, see alignment E=3e-09 PF13847: Methyltransf_31" amino acids 131 to 257 (127 residues), 42.3 bits, see alignment E=2.6e-14 PF13649: Methyltransf_25" amino acids 131 to 211 (81 residues), 35 bits, see alignment E=7.6e-12 PF01170: UPF0020" amino acids 151 to 206 (56 residues), 29.4 bits, see alignment E=2.6e-10

Best Hits

Swiss-Prot: 60% identical to PRMB_NEIG1: 50S ribosomal protein L3 glutamine methyltransferase (prmB) from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)

KEGG orthology group: K07320, putative adenine-specific DNA-methyltransferase [EC: 2.1.1.72] (inferred from 75% identity to mep:MPQ_0968)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p"

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (300 amino acids)

>GFF893 50S ribosomal protein L3 glutamine methyltransferase (Methylophilus sp. DMC18)
MTTNLHTIRDWLRYAVSRFENSDIFYGHGTDNSYDEAVWLIMSALHLPHDTLNNFLDARL
TTEESQHLATLIDKRVTEHTPTAYLVNEAWLRGFKFYVDERVIVPRSFIAELLEDGLQPW
VEFPEMVESAADICTGSGCLGVLLAEAFPNAHIDVVDISPDAIEVCNINIQRYGLEHQVQ
AVQSDMFTALKGRKYDVIISNPPYVDAPSMAELPEEYRKEPQLALGSGVAGLDHTHVLLT
QAAEYLNDGGVLVVEIGHNRDALHEAYPDLPFTWLDTSGGDQFVFMLTKEDLLEAGLHTE