Protein Info for GFF888 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 501 TIGR01722: methylmalonate-semialdehyde dehydrogenase (acylating)" amino acids 5 to 485 (481 residues), 644.2 bits, see alignment E=6.4e-198 PF00171: Aldedh" amino acids 16 to 481 (466 residues), 462.9 bits, see alignment E=5.2e-143

Best Hits

Swiss-Prot: 54% identical to BAUC_PSEAE: Putative 3-oxopropanoate dehydrogenase (bauC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 100% identity to ecq:ECED1_0300)

MetaCyc: 56% identical to malonate-semialdehyde dehydrogenase (acetylating) (Halomonas sp. HTNK1)
Malonate-semialdehyde dehydrogenase (acetylating). [EC: 1.2.1.18]

Predicted SEED Role

"Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27)" in subsystem Inositol catabolism (EC 1.2.1.27)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.18 or 1.2.1.27

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (501 amino acids)

>GFF888 Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
METVGNFIHGKTTLSSSGETLPVTNPATGKVIRQVTQSTREEMLAAIQSAHEAFPAWSKM
TPLRRARILFEFKVLLEKHRDELAALIVSEHGKVWSDALGELTRGIEVVEFACGIPHLSK
GEYSFNVGSGVDSFSLMQPLGVVAGITPFNFPAMVPMWMFPVALACGNTFVLKPPALVPS
ASLRMAQLLQEASLPDGVFNVVHCGNEAASLLTSDPRVQAVSFVGSSAVAEHIYTTASAH
GKRVQAFGAAKNHAIVMPDADLDATVNAIMGGAFGSAGERCMALPVVVAVGDETADRLIE
RLKPLIAALRIGPGELRGKDENEMGPVVSRAHQQKVLGYIDKGVSEGATLVMDGRNYSVA
GYPEGFYVGGTLFDNVTPEMTIWREEIFGPVLGIVRVPDYATAISTVNSHEFGNGSVIFT
TNGHYAREFAQSVEAGMVGINIPVPVPMAFHSFGGWKRSVFGALNVHGPDGVRFYTRMKT
VTSRWPNGQQIVSEFSMPTLG