Protein Info for GFF877 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Butyryl-CoA dehydrogenase (EC 1.3.99.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02771: Acyl-CoA_dh_N
  • amino acids 9 to 119 (111 residues), 131 bits, E=5.1e-42
• PF02770: Acyl-CoA_dh_M
  • amino acids 123 to 218 (96 residues), 99.4 bits, E=2.1e-32
• PF00441: Acyl-CoA_dh_1
  • amino acids 230 to 377 (148 residues), 152 bits, E=2.9e-48
• PF08028: Acyl-CoA_dh_2
  • amino acids 250 to 367 (118 residues), 51.3 bits, E=2.9e-17

Best Hits

Swiss-Prot: 55% identical to ACADL_PIG: Long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADL) from Sus scrofa

KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 68% identity to pmy:Pmen_0898)

MetaCyc: 52% identical to long-chain-acyl-CoA dehydrogenase monomer (Homo sapiens)
LONG-CHAIN-ACYL-COA-DEHYDROGENASE-RXN [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; RXN-21775 [EC: 1.3.8.8, 1.3.8.7]

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• adipate degradation (5/5 steps found)
• 2-methyl-branched fatty acid β-oxidation (11/14 steps found)
• fatty acid salvage (5/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Fatty acid metabolism
• Propanoate metabolism
• Valine, leucine and isoleucine degradation
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.2, 1.3.99.3

Use Curated BLAST to search for 1.3.8.7 or 1.3.8.8 or 1.3.99.2 or 1.3.99.3

Protein Sequence (378 amino acids)

>GFF877 Butyryl-CoA dehydrogenase (EC 1.3.99.2) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MLNRTLFGEEHELFRDQARRFLEEEVIPHHAGWENDGIVPRAVWRKAGAAGLLCPAIPEQ
YGGGGGTRLHSAVLIEEVSRAGTSGIGFGLHSDIVAPYILAYGTEAQKRNWLPKMATGEV
IGAIAMTEPGAGSDLQSVRTSAVRDGDELVINGQKTFITNGQNADVVIVVAKTDPTQGAK
GTSLVLVETNRPGFTRGRNLEKIGMKAQDTSELFFEDVRVPASNILGGDGKGFFLLMQEL
AWERMQIAIGAVAGAEAALEWTLAYTKDRKAFGQRVIDFQHNRFKLAEMKTEVQIAQVFV
DRLLGLLMDGQLDDATAAMAKYWTTDLHCKVIDQCLQMFGGFGYMWEYPIARAYADARVA
RIYGGANEIMKEIISRTL