Protein Info for GFF873 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 653 PF00289: Biotin_carb_N" amino acids 3 to 111 (109 residues), 155.3 bits, see alignment E=3e-49 PF02786: CPSase_L_D2" amino acids 117 to 323 (207 residues), 244.3 bits, see alignment E=3.8e-76 PF02222: ATP-grasp" amino acids 132 to 293 (162 residues), 30.2 bits, see alignment E=1.4e-10 PF07478: Dala_Dala_lig_C" amino acids 142 to 292 (151 residues), 41.1 bits, see alignment E=6e-14 PF02785: Biotin_carb_C" amino acids 337 to 443 (107 residues), 124.8 bits, see alignment E=6.4e-40 PF00364: Biotin_lipoyl" amino acids 583 to 647 (65 residues), 52 bits, see alignment E=2e-17 PF13533: Biotin_lipoyl_2" amino acids 585 to 620 (36 residues), 26 bits, see alignment 2.4e-09

Best Hits

KEGG orthology group: K13777, geranyl-CoA carboxylase alpha subunit [EC: 6.4.1.5] (inferred from 56% identity to pfl:PFL_4199)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4, 6.4.1.5

Use Curated BLAST to search for 6.4.1.4 or 6.4.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (653 amino acids)

>GFF873 Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MNFTKILIANRGEIACRIIRTAQALGYRTVAVYSEADAQALHVALADEAVCIGPAPVRES
YLNVAALLAAARSTGADAVHPGYGFLSENDGFAQACLDAGLVFIGPDPQAILKMGNKAGA
KRLMLAAGVPCVPGYQGTDQSDATLLTKAAEIGFPIMVKAAAGGGGRGMRLVEHPQDLAV
ALASARSEAGNAFGSEELILERAVIEPRHVEIQVFGDQHGHIIHLGERDCSIQRRHQKVF
EEAPSPAVTPQLREQMGAAAVAAARTVNYVGAGTVEFLLDSEGRFYFLEMNTRLQVEHPV
TECITGLDLVAWQIAVARGEHLPLTQEQVHLNGHAIEVRLYAEDPYNGFLPQSGDVALWC
PPTGEGVRVDHGLASGQTVSPHYDPMIAKIIVHGATRDEARRRLIGALQRTRLLGLPHNR
AFLEAVASHPAFAAGETTTAFIAQHFGAAALVRPQPDSLALALAGTLWFEASATPVAAAP
WRSSGPARWPMLIGEGEHRHAVHITVTGTHSYTLEVGAALHHITLSGREGECLHLIADGV
QQHAAAVFAGGVLHLELGDLSASFEDLTYDPPAAAAGSTESRVLAPMNGRVLAVDVQHGD
TVTKGQRLAVLEAMKMEHQLLARRDGVVDQVAVRAGDQVATRALLVSLVEQPD