Protein Info for GFF87 in Sphingobium sp. HT1-2

Annotation: Kup system potassium uptake protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 640 transmembrane" amino acids 29 to 48 (20 residues), see Phobius details amino acids 66 to 86 (21 residues), see Phobius details amino acids 120 to 141 (22 residues), see Phobius details amino acids 156 to 175 (20 residues), see Phobius details amino acids 186 to 207 (22 residues), see Phobius details amino acids 229 to 251 (23 residues), see Phobius details amino acids 263 to 285 (23 residues), see Phobius details amino acids 306 to 331 (26 residues), see Phobius details amino acids 356 to 376 (21 residues), see Phobius details amino acids 383 to 406 (24 residues), see Phobius details amino acids 413 to 432 (20 residues), see Phobius details amino acids 442 to 460 (19 residues), see Phobius details PF02705: K_trans" amino acids 30 to 481 (452 residues), 617.6 bits, see alignment E=1.1e-189 PF22776: K_trans_C" amino acids 492 to 640 (149 residues), 184.7 bits, see alignment E=9.6e-59

Best Hits

Swiss-Prot: 70% identical to KUP2_SPHWW: Probable potassium transport system protein kup 2 (kup2) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K03549, KUP system potassium uptake protein (inferred from 92% identity to sjp:SJA_C1-17450)

MetaCyc: 48% identical to K+:H+ symporter Kup (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-3

Predicted SEED Role

"Kup system potassium uptake protein" in subsystem Potassium homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (640 amino acids)

>GFF87 Kup system potassium uptake protein (Sphingobium sp. HT1-2)
MADDSAVAVPEDGEQDGHGHARQKATAKLVVGAIGIVFGDIGTSPLYAFRETFAGHHHLD
LDPDHILGVISLMFWSMMLVVTLKYVSIIMRADNKGEGGSLALLALINGQTKTQRWSRGI
VLLGVFATALFYGDSMITPAVSVLSAVEGLAVYNANLAPAILPVAVLILLGLFWIQGLGT
NKVATLFGPIMLIYFVTIASLGVLSIVKTPGILYAFNPYWAVMFFATDPLPAFLALGSVV
LAVTGAEALYADMGHFGRSPIRVSWLIFVLPALMLNYMGQGALLFREGAAALHSPFYNLA
PQWMQLPLIGLATLAAIIASQAVISGAFSVTQQAIQLGFMPRLRIEHTSASTAGQIYIPL
INWGLMVMVILLVLVFKTSSNLTAAYGIAVTGAMFIDNVLLTVVLYRLWHWKWYYAAPLL
AVFYLVDGAYLAANLTKVPDGGWFPLLIGFVVFTLLTTWSRGRRLVQERLREAAMPIPVF
VASAANSAVRVPGTAVFMTSTPDGVPHALLHNLKHNKVLHERVILLTVKIKDVPVVEDDG
RCKLEDLGRGFFRLVLQYGFMQEPDVPAALKNVTGCGQAFKMMDTSFFLARQTLLPSANP
GMPLWREKIFAWMLRNAESAMEFFRLPTNRVVELGSQVEI