Protein Info for GFF861 in Methylophilus sp. DMC18

Annotation: Ribonuclease G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 490 TIGR00757: ribonuclease, Rne/Rng family" amino acids 15 to 426 (412 residues), 500.7 bits, see alignment E=1.7e-154 PF00575: S1" amino acids 40 to 117 (78 residues), 24.8 bits, see alignment E=2.3e-09 PF10150: RNase_E_G" amino acids 122 to 393 (272 residues), 329.6 bits, see alignment E=1.6e-102

Best Hits

Swiss-Prot: 56% identical to RNG_SHIFL: Ribonuclease G (rng) from Shigella flexneri

KEGG orthology group: None (inferred from 95% identity to rcu:RCOM_0450990)

MetaCyc: 56% identical to RNase G (Escherichia coli K-12 substr. MG1655)
Ribonuclease E. [EC: 3.1.26.12]

Predicted SEED Role

"Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-)" in subsystem Bacterial Cell Division or RNA processing and degradation, bacterial (EC 3.1.4.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.26.12, 3.1.4.-

Use Curated BLAST to search for 3.1.26.12 or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (490 amino acids)

>GFF861 Ribonuclease G (Methylophilus sp. DMC18)
MSLAQEVLINVTPQETRVAILEQGIVQELHIERASSLGIVGNIYRGTVVRVLPGMQSAFV
EIGLPRAAFLHAADIMECHTEEGAVKTDRPIQEVLHEGQSIIVQVIKEAIGTKGARLTTE
ISIAGRFLVYLPYQKHIGVSQRIDHEEEREFLRNRLLGLLPEAREGGYIIRTMSENATDQ
ELLADIEYLRKVWQKIQTESTKVGERSLIFYDLSLPRRILRDVVCTETTSIRVDSREMFE
RLKEFAELYVSHAVKPLTHYKGERSLFEFYDIENEIEKALARKVELKSGGYLIFDQTEAL
TTVDVNTGSFVSGKNLSDTIFKTNLEAAQAIARQLRLRNLGGIIIIDFIDMDEDAQRAAV
LEELRSAVTLDRARINVNGFSALGLVELTRKRTRESLAHLLCESCQVCQGRGELKTAQTI
CYEIMRELLRESKQFKNAKEFKILASPEVINMLGDEESQSLANLSDFIKRPVSLHPDTQY
GREEFDIVWV