Protein Info for GFF860 in Variovorax sp. SCN45

Annotation: Cocaine esterase (EC 3.1.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 613 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR00976: hydrolase CocE/NonD family protein" amino acids 57 to 574 (518 residues), 180.8 bits, see alignment E=3e-57 PF02129: Peptidase_S15" amino acids 57 to 307 (251 residues), 115.7 bits, see alignment E=3.2e-37 PF08530: PepX_C" amino acids 333 to 571 (239 residues), 126.7 bits, see alignment E=1.3e-40

Best Hits

KEGG orthology group: K06978, (no description) (inferred from 66% identity to dac:Daci_4534)

Predicted SEED Role

"Cocaine esterase (EC 3.1.1.-)" (EC 3.1.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.-

Use Curated BLAST to search for 3.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (613 amino acids)

>GFF860 Cocaine esterase (EC 3.1.1.-) (Variovorax sp. SCN45)
MAPSLVFAAGLAGCGGGSSGTAIIPVPVAVGGSTPPPAAPQVNEGTVRKWGYLTLPDGNK
MRYTVLLPRATGTFPVLIEYDGYGSGVVADEAPNWVPEGYAVMGLNVPGTGCSSGDDQVF
DATVGAAGAFAVEWAAQQPWSSGKVGMVGYSYPGYNQLWVAAQRPKGLVAIAPGKNVTDP
YRDVGYPGGIQNIGFPAAWWGRFPDIWRTSAEDAARLDGDTECARNVRDNIEKIKRPDLD
LVNWLDNDPHYGARYAKKSAILVTGNIDIPTLGTQSWQDEQVGPRMGYYEDTIAPEKMWL
ISSNGLHGTSDFSAYMRDVQRRFYAHFLKGENNGFEREPHVHLLQEMQETVGEKDSLTLV
HTAAATFDRLPVKTRPMRLWLQPGGQLTDKAPADDRTPSSSYAYPIESPAVNDQDKSSWA
AVSAPEGQQVFTTAPLPQDLSFYGEGSADLYLSSTATDTDLQVSLSEVRPDGMEMFVQRG
WLRASKRQLDDAKSTVLRPTGDFTAATVKMLEPDTPVLMRLELQKFAHVFRAGSSLRVTI
DTPSQTGYWVFGIRQTPATNRIWHDTARASSIVLGHVAYPHAPGLPTCGIVQQQPCRKNE
IAVPSGVGPKPPV