Protein Info for GFF85 in Pseudomonas sp. DMC3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 644 PF00571: CBS" amino acids 180 to 234 (55 residues), 43.5 bits, see alignment 6.6e-15 amino acids 246 to 295 (50 residues), 37 bits, see alignment 7e-13 PF03445: DUF294" amino acids 323 to 462 (140 residues), 145.8 bits, see alignment E=1.6e-46 PF10335: DUF294_C" amino acids 499 to 642 (144 residues), 147.7 bits, see alignment E=4.4e-47

Best Hits

KEGG orthology group: K07182, CBS domain-containing protein (inferred from 96% identity to pfo:Pfl01_5185)

Predicted SEED Role

"Predicted signal-transduction protein containing cAMP-binding and CBS domains" in subsystem cAMP signaling in bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (644 amino acids)

>GFF85 hypothetical protein (Pseudomonas sp. DMC3)
MSKADAFTQAGKTAVLQNIQGTLQFLQRFPPFNQMEHAHLAYLVEQCQLRFFAQGESIIK
PADGPVEHFHIVKQGRVVGERQHITKPGTETTFEITTGECFPLAALLGERATRTEHLAGE
DTFCLQLNKLAFIKLFALSSAFRDFALRGVSSLLDQVNQQVQQKAVETLGTQYSLNTRLG
ELAMRHPVTCSAETPLREAVKLMHEQQVGSIVAVDEHKAPLGIFTLRDLRHVVAEGVGDF
SDAIEQHMTRSPFYLSPDHSAFDAAIAMTERHIAHVCLVKDQRLCGVVSERDLFSLQRVD
LVHLARTIRSAQRVEQLVTLRGEIGQLVERMLAHGASSTQITHIITLLNDHTVCRVIELT
LAEKGDPGVPFSWLCFGSEGRREQTLHTDQDNGILFDARDAAHAAEIRGKLLPIAQQINQ
SLAQCGFTLCKGNIMAGNPELCLSRAEWARRFAAFIREATPENLLGSSIYFDLRVVWGDE
QGCEQLRRGILDQVADNRLFQRMLAENALRNRPPVGRFREFVLARKNGEKATLDLKIQGL
TPFVDGARLLALANGIDANNTLERFRQLVDKEVIEPLDGAAYEEAYHFIQQTRMQQHQLQ
TRENQPYSNRVDPDSLNHLDRRILRESLRQAQRLQSSLTLRYQL