Protein Info for GFF839 in Variovorax sp. SCN45

Annotation: Retron-type RNA-directed DNA polymerase (EC 2.7.7.49)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 648 transmembrane" amino acids 591 to 617 (27 residues), see Phobius details amino acids 625 to 646 (22 residues), see Phobius details PF00078: RVT_1" amino acids 192 to 404 (213 residues), 80.6 bits, see alignment E=6.6e-27

Best Hits

Predicted SEED Role

"Retron-type RNA-directed DNA polymerase (EC 2.7.7.49)" (EC 2.7.7.49)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (648 amino acids)

>GFF839 Retron-type RNA-directed DNA polymerase (EC 2.7.7.49) (Variovorax sp. SCN45)
MTMQSPPASSIPDSALTREQLYERIRKGSKQEVILEEMQRLGFWPQEAAQPTVEVQLIRR
EGELQAALSKLGDELRGIEDRDKALKTMRKERMAKARERREETRQRLAQDRHARAIAWHE
RRQRELLYVGEGVSGGLGEAQSDAEVLARNALPALHHAGDLAQAMGIGLGELRFLAWHRD
VANVSHYQRFTIAKKSGGERHISAPMPRMKRAQYWVLDNILAKMPVHDAVHGFLPGRSIL
TNAAPHVGQDVVINLDLKDFFPSIGMRRVRGVFRQLGYSSQVASLLALVCTEAPTDEVQL
DGRRYFVARGERVLPQGAPTSPMLTNLLCRRLDARLAASAAKLGFRYTRYADDLSFSAGP
AHSRDTAKLLWRVKQIVASEGLTVHPDKQQVMRRHRQQHVTGIVVNDKLSLDRDTMRRFR
AVLHQAESRGPQGLRWNGNSDVIGALRGYANFIAMTDAARGAPYVERVRALAAKHGDGKA
TAPAVQQTQPSQRSLPAGQFRKQSAAGQAPWPEWWQPAQAPAPILEKTAEQIAEEKKAQR
EAARAQAAPPAVARPAQTQQAPAAQPAATTSTPAAPGALSHIGWIASMMFQALYVVTTFL
AGSSPLIWLLTGGVFISNFLRRKSGWGGFLAAVVVSSAVASLVHSMRK