Protein Info for PS417_04240 in Pseudomonas simiae WCS417

Annotation: glutamyl-tRNA amidotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 99% identical to GATA_PSEFS: Glutamyl-tRNA(Gln) amidotransferase subunit A (gatA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 99% identity to pfs:PFLU0861)

MetaCyc: 54% identical to glutamyl-tRNAGln amidotransferase subunit A (Bacillus subtilis)
Glutaminyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7]

Predicted SEED Role

"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)

MetaCyc Pathways

L-citrulline biosynthesis (8/8 steps found)
superpathway of L-citrulline metabolism (10/12 steps found)
L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
L-glutamate and L-glutamine biosynthesis (6/7 steps found)
ammonia assimilation cycle III (3/3 steps found)
L-glutamate biosynthesis I (2/2 steps found)
L-glutamine degradation I (1/1 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7

Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TW46 at UniProt or InterPro

Protein Sequence (483 amino acids)

>PS417_04240 glutamyl-tRNA amidotransferase (Pseudomonas simiae WCS417)
MHHMTLAEIARGLADKTFSSEELTKTLLARIAEHDPKVNSFISLTEELALSQAKAADARR
ANGESGALLGAPIAHKDLFCTQGIRTSCGSKMLDNFKAPYDATVVSKLAAAGAVTLGKTN
MDEFAMGSANESSYYGAVKNPWNLDHVPGGSSGGSAAAVAARFLPAATATDTGGSIRQPA
AFTNLTGLKPTYGRVSRWGMIAYASSLDQGGPLARTAEDCAILLQGMAGFDKQDSTSIDE
PVPDYSASLNTSLKGLRIGVPKEYFSAGLDPRIAELVHNSVKTLESLGAVIKEISLPNNQ
HAIPAYYVIAPAEASSNLSRFDGVRFGYRCENPKDLTDLYKRSRGEGFGAEVQRRIMVGA
YALSAGYYDAYYLKAQKIRRLIKNDFMAAFEEVDVILGPTTPNPAWKIGAKTGDPIAEYL
EDLYTITANLAGLPGLSMPAGFVDGLPVGVQLLAPYFQEGRLLNVAHQYQLNTDWHTRTP
TGF