Protein Info for PS417_04220 in Pseudomonas simiae WCS417

Annotation: 4-carboxymuconolactone decarboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 46% identical to DC4C_ACIAD: 4-carboxymuconolactone decarboxylase (pcaC) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K01607, 4-carboxymuconolactone decarboxylase [EC: 4.1.1.44] (inferred from 96% identity to pfs:PFLU0857)

Predicted SEED Role

"4-carboxymuconolactone decarboxylase (EC 4.1.1.44)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 4.1.1.44)

MetaCyc Pathways

aromatic compounds degradation via β-ketoadipate (9/9 steps found)
protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
toluene degradation III (aerobic) (via p-cresol) (8/11 steps found)
superpathway of aromatic compound degradation via 3-oxoadipate (21/35 steps found)
superpathway of aerobic toluene degradation (12/30 steps found)

KEGG Metabolic Maps

Benzoate degradation via hydroxylation

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.44

Use Curated BLAST to search for 4.1.1.44

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U706 at UniProt or InterPro

Protein Sequence (126 amino acids)

>PS417_04220 4-carboxymuconolactone decarboxylase (Pseudomonas simiae WCS417)
MTDQKKPGVEMRRQVMGDVFVDRALGNATEFTQPLQDFVNEHAWGSVWNREGLPLKTRSL
ITLAALTALKCPQELKGHVRGALNNGCTVEEIREALLHCAVYAGVPAAIDAFRAAQEVIE
TYQKEG