Protein Info for GFF830 in Methylophilus sp. DMC18

Annotation: Metalloprotease PmbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 PF01523: PmbA_TldD_1st" amino acids 33 to 89 (57 residues), 49.4 bits, see alignment 6.1e-17 PF19290: PmbA_TldD_2nd" amino acids 117 to 223 (107 residues), 80.1 bits, see alignment E=2.4e-26 PF19289: PmbA_TldD_3rd" amino acids 230 to 437 (208 residues), 232 bits, see alignment E=7.2e-73

Best Hits

Swiss-Prot: 52% identical to PMBA_ECOLI: Metalloprotease PmbA (pmbA) from Escherichia coli (strain K12)

KEGG orthology group: K03592, PmbA protein (inferred from 72% identity to mmb:Mmol_1894)

MetaCyc: 52% identical to metalloprotease subunit TldE (Escherichia coli)
3.4.24.-

Predicted SEED Role

"TldE protein, part of TldE/TldD proteolytic complex"

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (440 amino acids)

>GFF830 Metalloprotease PmbA (Methylophilus sp. DMC18)
MQADQLQQIAQDIIRLTQTAGASSAETEVSFGMGQSVSVRQGETENIEYNRDKGCSVTVY
FGQQKGYASTSDLSPQALRDTVEAACNIAKYTAQDPFCGLADADLMATSFPDLSLHHPWQ
VDVDAALALAKRCESAALAVDHRISNSEGASVYTQSGAFAYANSHGFIGGYASSRHSISC
SVIAEADGLMQRDYWYSSARDALDLASPEQVGQIAGERTARRLGARPIKTGQYPVLFEAP
LASGLIGTLMSAISGGNLYRKSSFLLDSLGQTIASPLLQIEDHPFLLKGAASSAFDSEGV
ACQTRTLVENGVLQGYLLGSYSARKLGLKTTGNAGGAHNILVRSTGQSFEQLLQTLGTGL
LVTELLGHGLNMVTGDYSRGASGYWVENGVIVHPVEEITIASNMKDMLKSIVAIGNDVIP
NSSKLTGSILIERMTVASSE