Protein Info for GFF822 in Variovorax sp. SCN45

Annotation: Na+/H+-dicarboxylate symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 447 transmembrane" amino acids 18 to 37 (20 residues), see Phobius details amino acids 51 to 73 (23 residues), see Phobius details amino acids 85 to 108 (24 residues), see Phobius details amino acids 158 to 176 (19 residues), see Phobius details amino acids 194 to 217 (24 residues), see Phobius details amino acids 234 to 254 (21 residues), see Phobius details amino acids 316 to 349 (34 residues), see Phobius details amino acids 352 to 352 (1 residues), see Phobius details amino acids 359 to 383 (25 residues), see Phobius details PF00375: SDF" amino acids 18 to 410 (393 residues), 386.7 bits, see alignment E=6.2e-120

Best Hits

Swiss-Prot: 87% identical to DCTA1_POLNA: C4-dicarboxylate transport protein 1 (dctA1) from Polaromonas naphthalenivorans (strain CJ2)

KEGG orthology group: K11103, aerobic C4-dicarboxylate transport protein (inferred from 94% identity to vpe:Varpa_3855)

MetaCyc: 74% identical to C4 dicarboxylate/orotate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-121; TRANS-RXN-121A; TRANS-RXN-121C; TRANS-RXN-122A; TRANS-RXN0-451; TRANS-RXN0-517; TRANS-RXN0-553

Predicted SEED Role

"Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (447 amino acids)

>GFF822 Na+/H+-dicarboxylate symporter (Variovorax sp. SCN45)
MESTTSTSARKLPLYRSLYVQVITAVIIGVLLGHFYPAVGEAMKPLGDGFIKLIKMIIAP
IIFCTVVVGIAGMEDMKKVGKTGGLALLYFEVVSSIALVVGLVLVNVLQPGAGMNVDPAT
LDTKSIASYTGPGKMTGTVDFILNIIPNTLVDAFAKGEILQVLLIAVLFGFALHRFGGRG
TLVFDVIEKGSHVLFGIVNYIMKLAPIGAFGAMAFTIGKYGLGSLFSLGKLMGTFYLTCL
LFIFVVLGLIARFHGFSIWKFIKYIKEELLIVLGTSSSESVLPRMMEKMENLGANKTCVG
LVIPTGYSFNLDGTSIYLTMAAVFIAQATNTHMTLVQEITLLAVLLLTSKGAAGVTGSGF
IVLAATLSAVGHVPVAGLALILGIDRFMSEARALTNLIGNGVATIVVAKWTNELDENRLH
AALNNETWIEAQEPEALDAARSGKMAG