Protein Info for GFF822 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868
Annotation: N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to AMIB_SALTY: N-acetylmuramoyl-L-alanine amidase AmiB (amiB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
KEGG orthology group: K01448, N-acetylmuramoyl-L-alanine amidase [EC: 3.5.1.28] (inferred from 99% identity to spt:SPA4175)MetaCyc: 84% identical to N-acetylmuramoyl-L-alanine amidase B (Escherichia coli K-12 substr. MG1655)
N-acetylmuramoyl-L-alanine amidase. [EC: 3.5.1.28]
Predicted SEED Role
"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)
MetaCyc Pathways
- peptidoglycan recycling I (14/14 steps found)
- peptidoglycan recycling II (7/10 steps found)
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.28
Use Curated BLAST to search for 3.5.1.28
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (439 amino acids)
>GFF822 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868) MIYRIKNAVIAALILLCAQAGAASLSDIQVSNGEQQARITLSFIGEPEYAYSQDGKRTVA LDIRQTGVIQGLPLQFSGNNLVKTIRAGTPKDAQSLRLLVDLTENGKTEAVKRQNGGNYT VIFTINADVPPPPPPVVAKRVESAPRPTEPARNPFKSSDDRLTGVTSSNTVTRPAARASA GAGDKVVIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFKGVLTRDGDY FISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANSEMANWLEQHEKQS ELLGGAGDVLANSQSDPYLSQAVLDLQFGHSQRVGYDVATNVLSQLDGVGSLHKRRPEHA SLGVLRSPDIPSILVETGFISNHGEERLLASDRYQQQIADAIYRGLRKYFAAHPIQSAPQ GGPGQTASTNQPGAITAAN