Protein Info for GFF817 in Variovorax sp. SCN45
Annotation: Similar to citrate lyase beta chain, 3
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01644, citrate lyase subunit beta / citryl-CoA lyase [EC: 4.1.3.34 4.1.3.6] (inferred from 86% identity to vap:Vapar_2028)Predicted SEED Role
"Citrate lyase beta chain (EC 4.1.3.6)" (EC 4.1.3.6)
MetaCyc Pathways
- citrate degradation (2/2 steps found)
- reductive TCA cycle II (7/12 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.1.3.34, 4.1.3.6
Use Curated BLAST to search for 4.1.3.34 or 4.1.3.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (292 amino acids)
>GFF817 Similar to citrate lyase beta chain, 3 (Variovorax sp. SCN45) VSGISLAPLAPLALARAFLFVPADRPERHVRALATGAGGVIVDLEDAVAPERKVAARDGL AASFATLPAEQRGRLLVRVNAQGTSWHGDDCARVGELARQGLIAGVMLPKAERAEDLARL AEAVGPRGVLVPLIESAAGLAAIDELAAAPQVLRLAFGNLDFQADLGLACGPDEAELVPV RLALLLATRRAGLPAPIDGVTPDWRDAGRLAADTARARRGGFGAKLCIHPDQVAPVHAAL GPSAEELAWARRVIEAIRASGGGVVSLDGRMVDAPVVRLAERLLALDGPPSL