Protein Info for GFF806 in Variovorax sp. SCN45

Annotation: Maleylacetoacetate isomerase (EC 5.2.1.2) @ Glutathione S-transferase, zeta (EC 2.5.1.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02798: GST_N
  • amino acids 1 to 74 (74 residues), 47.2 bits, E=3.6e-16
• TIGR01262: maleylacetoacetate isomerase
  • amino acids 2 to 210 (209 residues), 260.6 bits, E=5e-82
• PF13417: GST_N_3
  • amino acids 3 to 80 (78 residues), 51 bits, E=2.2e-17
• PF13409: GST_N_2
  • amino acids 9 to 75 (67 residues), 49.1 bits, E=1.1e-16

Best Hits

Swiss-Prot: 48% identical to NAGL_RALSP: Maleylpyruvate isomerase (nagL) from Ralstonia sp.

KEGG orthology group: K01800, maleylacetoacetate isomerase [EC: 5.2.1.2] (inferred from 89% identity to vpe:Varpa_3871)

MetaCyc: 46% identical to glutathione transferase subunit (Escherichia coli B)
Glutathione transferase. [EC: 2.5.1.18]

Predicted SEED Role

"Maleylacetoacetate isomerase (EC 5.2.1.2) @ Glutathione S-transferase, zeta (EC 2.5.1.18)" (EC 2.5.1.18, EC 5.2.1.2)

MetaCyc Pathways

• L-tyrosine degradation I (5/5 steps found)
• 4-hydroxy-2-nonenal detoxification (1/4 steps found)
• pentachlorophenol degradation (5/10 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• gliotoxin biosynthesis (1/9 steps found)
• glutathione-mediated detoxification II (1/9 steps found)
• indole glucosinolate activation (intact plant cell) (3/12 steps found)
• camalexin biosynthesis (2/12 steps found)

KEGG Metabolic Maps

• Drug metabolism - cytochrome P450
• Glutathione metabolism
• Metabolism of xenobiotics by cytochrome P450
• Styrene degradation
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.18, 5.2.1.2

Use Curated BLAST to search for 2.5.1.18 or 5.2.1.2

Protein Sequence (212 amino acids)

>GFF806 Maleylacetoacetate isomerase (EC 5.2.1.2) @ Glutathione S-transferase, zeta (EC 2.5.1.18) (Variovorax sp. SCN45)
MKLHNYFRSSSSFRVRIALNLKGLDYEYVPVHIARGEHRTGSYPALSADMLVPLLEDDGE
RFSQSMAIMEYLDELHPEPPLLPSDPAGRAHVRALSQSIACEIHPLNNLRVLKYLVKDLK
LDEEAKNNWYRHWVRDGMLAFERQLAQQPASTYCWGNTPTMADCCLVPQIFNGRRFDCDF
SGLPRTMAAFEACMELDAFQRAQPSRAPDAEA