Protein Info for GFF799 in Xanthobacter sp. DMC5

Annotation: Vitamin B12 import ATP-binding protein BtuD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 336 PF00005: ABC_tran" amino acids 30 to 180 (151 residues), 113.4 bits, see alignment E=1.3e-36 TIGR01727: oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal domain" amino acids 230 to 319 (90 residues), 82.5 bits, see alignment E=8.7e-28 PF08352: oligo_HPY" amino acids 232 to 299 (68 residues), 65.5 bits, see alignment E=4.8e-22

Best Hits

Swiss-Prot: 52% identical to Y3864_BRUME: Putative peptide import ATP-binding protein BMEII0864 (BMEII0864) from Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)

KEGG orthology group: K02032, peptide/nickel transport system ATP-binding protein (inferred from 58% identity to azl:AZL_a10300)

MetaCyc: 51% identical to dipeptide ABC transporter ATP binding subunit DppF (Escherichia coli K-12 substr. MG1655)
ABC-8-RXN [EC: 7.4.2.9]

Predicted SEED Role

"Oligopeptide transport ATP-binding protein OppF (TC 3.A.1.5.1)" in subsystem ABC transporter oligopeptide (TC 3.A.1.5.1) (TC 3.A.1.5.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (336 amino acids)

>GFF799 Vitamin B12 import ATP-binding protein BtuD (Xanthobacter sp. DMC5)
MSAPVETPALEVRDLVVSYRTGRGPLSAVDGVSFAIRPGETLGLVGESGCGKSSLAKAIV
QLVEPTSGALKVEGHDITGLTARARRALRPKVQMVFQDPMGALDPRQRAGTILETPLKLN
GVRGKAARRARVASLMEAVGLRPEFIDRLPHEFSGGQRQRIGIARALALDPAVIVCDEPV
SALDVSLQAQVLNLLRKVQRETSVAYLFISHDLSVVEHIADRVAIMYLGRIVELAPRETL
WHAPLHPYTRALIEALPRMDPGASRIADKTIVGGDLPSPFAPPKGCRFHTRCPIAVGRCA
IDAPALSDAGDGHLVACHLAPGAVAAPTAGAVLQPA