Protein Info for GFF795 in Variovorax sp. SCN45
Annotation: 5-methyltetrahydropteroyltriglutamate--homocystei ne methyltransferase (EC 2.1.1.14)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 83% identical to METE_BURL3: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)
KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 86% identity to mms:mma_0750)Predicted SEED Role
"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)
MetaCyc Pathways
- aspartate superpathway (24/25 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
- superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
- superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
- superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
- L-methionine biosynthesis III (4/4 steps found)
- S-adenosyl-L-methionine salvage II (3/3 steps found)
- L-methionine biosynthesis II (5/6 steps found)
- L-methionine biosynthesis I (4/5 steps found)
- S-adenosyl-L-methionine salvage I (3/4 steps found)
- seleno-amino acid biosynthesis (plants) (3/5 steps found)
- superpathway of seleno-compound metabolism (8/19 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.1.1.14
Use Curated BLAST to search for 2.1.1.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (763 amino acids)
>GFF795 5-methyltetrahydropteroyltriglutamate--homocystei ne methyltransferase (EC 2.1.1.14) (Variovorax sp. SCN45) MTTTHNLGFPRIGAKRELKFALESYWKGESSRDALKALGAQLRQRHWNDQAGIDLVPVGD FAFYDQVLDMSFTLGNLPERVRGFHGDALDNYFRVARGRSAQGAEEHAGCCGGVAAGEMT KWFDTNYHYIVPEFTAQTEFRLDASRLLEQLAEAKAQGVKAKPVLVGPVTYLSIGKAKDD SDKLALLPRLLQVYAELLETLAAQGVEWVQIDEPLLVTELDADWQHAFNTAYHQLKASRI KILIATYFGQLQENKYLAANLPVAGLHVDAINGRDDIVPLLTMLPAHKVLSLGVINGRNI WKSDLAFILDWLEPLAARLGDRLWIAPSCSLLHVPVDLASEQKLDTEVKSWLAYALQKLE ELRVLATALRDGRDAVKDALAANSAALAARRASPRVNNPAVQAAVAKLTSHLGQREGAYA KRAAKQAAFLKLPKFPTTTIGSFPQTAEIRHARSEYKAGRLDDAGYKAAMQAEIARSVRE QEALDLDVLVHGEAERNDMVEYFGEQLEGYAFSQFGWVQSYGSRCVKPPILFGDISRPKA MTVEWIQYAQSLTQRPMKGMLTGPVTILNWSFVRDDQPRSASCKQLALAIREEVLDLEKG GVRVIQIDEAALREGLPLRKSQWQEYLDWAVESFRITANGVRDETQIHTHMCYSEFNDII ASIADMDADVITIETSRSDMELLDAFDDFKYPNEIGPGVYDIHSPNIPSQEHIVQLMKKA AERVPAERLWVNPDCGLKTRQWVEVLPALTNMVAAAKTLRTSA