Protein Info for GFF768 in Variovorax sp. SCN45

Annotation: Trehalose synthase (EC 5.4.99.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1114 TIGR02456: trehalose synthase" amino acids 23 to 558 (536 residues), 924.2 bits, see alignment E=2.3e-282 PF00128: Alpha-amylase" amino acids 46 to 399 (354 residues), 253.6 bits, see alignment E=5.8e-79 PF16657: Malt_amylase_C" amino acids 478 to 555 (78 residues), 94.1 bits, see alignment 6.9e-31 TIGR02457: putative maltokinase" amino acids 593 to 1109 (517 residues), 441.2 bits, see alignment E=5.6e-136 PF18085: Mak_N_cap" amino acids 620 to 695 (76 residues), 22.8 bits, see alignment 2.4e-08

Best Hits

KEGG orthology group: K05343, maltose alpha-D-glucosyltransferase [EC: 5.4.99.16] (inferred from 76% identity to aav:Aave_2022)

Predicted SEED Role

"Trehalose synthase (EC 5.4.99.16)" in subsystem Trehalose Biosynthesis (EC 5.4.99.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1114 amino acids)

>GFF768 Trehalose synthase (EC 5.4.99.16) (Variovorax sp. SCN45)
MNAPVSHIALETVEIDNSDDPLWYRDAVIYQLNVKAFFDSNNDGMGDFQGVTAKLDYVKE
LGVNTIWLMPFYPSPLRDDGYDISEYEDVHPQYGTLADFREMLAEAHKRGLRVITELVIN
HTSSEHPWFKAARLAPPGSPERDFYVWSDTDQLYQGTRIIFTDTETSNWTWDPVAKQYYW
HRFFSHQPDLNFDNPAVMEAIFKTMRFWLDMGVDGFRLDAIPYLVERDGTSNENLPETHA
VIKRLRAAIDAQYKNRFLLAEANMWPEDVREYFGDGDECHMAYHFPLMPRMYMAIAQEDR
HPIVEIMAQTPDIPEGCQWAIFLRNHDELTLEMVTSKERDYMYTMYAADMRARINLGIRR
RLAPLMENDLDRVKLMNGMLLSMPGSPIIYYGDEIGMGDNVFVGDRNGVRTPMQWSPDRN
AGFSRADPQRLYLQPIMDAMYGYEALNVETQARDASSLLNWTKRMLAVRKTSHAFGRGRR
RFLKPGNRKILAYLSEYDGDVILAVFNLSRAAQPVELDLSEFKGHVPIEMLGRAPFPPIG
ELPYLLTLSSYGFYWFKLTAEADAPSWHDQGVALQEWPTLVLFDGWTSFFRDRVMPWRIG
MSERMRSQFELETLPRHIEIQRWYASKGTAIERARIVDHAVWEVNGQSWMLPLLALDGPP
GGATYFMPLALAWEERDEERMAGVAQAALAKVRQQAQVGLMGDAFYDEAFCRELVRAIGQ
GAELATGGGKLVFKPTAAFARLGIDLDALPVGRPSGVSSNTVVTLDETLFLKGYRHVREG
INPELEMGRFLTEVARYPHCVPVLGALEYMSADGRTMTLAMVQSYVANQGDGWDYTLGYL
ERFLRDVATTDGAVDAPDVLAVHGGFLALIGTLGRRTAELHRALATRNGAAAFDPEPLSA
TDVAGYRTHAAGDATATMALLRERIGTLPPAAQADAEALLRAADTLQANIAARRFVGEGG
GGAALKSRYHGDYHLGQVLVKDNDFIIIDFEGEPARSFEERRAKSSPLRDVAGMLRSFNY
ARWSALRRVAQSPEEAEKLAAPAIAWEQATRDAFMKGYAGTLAQPDGAPIDGELLALFEL
EKALYELRYELNNRADWVQVPLHGVLALIQPNGA