Protein Info for GFF767 in Variovorax sp. SCN45

Annotation: Alpha-amylase (EC 3.2.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 689 PF11896: GlgE_dom_N_S" amino acids 30 to 217 (188 residues), 163.5 bits, see alignment E=6.6e-52 PF21702: GLGE_C" amino acids 587 to 675 (89 residues), 97 bits, see alignment E=6.4e-32

Best Hits

KEGG orthology group: None (inferred from 88% identity to vpe:Varpa_3004)

Predicted SEED Role

"Alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (689 amino acids)

>GFF767 Alpha-amylase (EC 3.2.1.1) (Variovorax sp. SCN45)
MKNIFSTRNAASVAPDASAGTDTNPGGNVRAVIDAVLPAVDNGRFAVKCVAGERVRVRAH
CFTDGHDVLRVQLCWRAQDQAEFREVPMKPLGNDVWEAVFSPPAIGRYVYTAVAWVDPFE
SWRSEMTRRVDPDDVRIASEVGAAEVAAAAARAEGADRIALQRWATELEAVAAAPGTDAV
SLKALALDEELAELARRHPDRRHAVRHPVELPLVADRERARFSTWYELFPRSAGMEPGVH
GTFKDVEARLPAIAGMGFDVLYFPPIHPIGRMQRKGPNNKLVSGPEDVGSPWAIGAAEGG
HKDILPALGTAKDFRRLLARAADHGLEIALDIAFQCAPDHPYVTAHPDWFRWRPDGTVQY
AENPPKKYQDIYPFNFESEDWRGLWAELKSVLDHWVGEGVRIFRVDNPHTKAFPFWEWAI
GEVKREHPDVIFLAEAFTRPKVMHRLAKLGFSQSYTYFTWRNTKEELVEYFTELSTAPGI
DYFRPNVWPNTPDILHEQLQHGDAPTYMARLVLAATLSASYGIYGPAYELREHLPREPGS
EEYLDSEKYQLRHWNHDDPESLAPFIARVNRIRRENPALHGDRTLRFLHIDNPQLLAYAK
ASADGANVVVTVVNLDPHNAQWGWLGLEPGSVGVNPPSRVFQMHDLLSGQRFQWQGDWHY
VRLDPHSCPAHVFVVRRRHGDERDFDYFL