Protein Info for GFF766 in Variovorax sp. SCN45

Annotation: 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 636 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 14 to 630 (617 residues), 895.2 bits, see alignment E=1.1e-273 PF02922: CBM_48" amino acids 30 to 111 (82 residues), 60.7 bits, see alignment E=2.1e-20 PF00128: Alpha-amylase" amino acids 179 to 249 (71 residues), 38.9 bits, see alignment E=9.8e-14 PF02806: Alpha-amylase_C" amino acids 531 to 631 (101 residues), 78.7 bits, see alignment E=5.4e-26

Best Hits

Swiss-Prot: 79% identical to GLGB_RHOFT: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Rhodoferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 89% identity to vpe:Varpa_3003)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.18

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (636 amino acids)

>GFF766 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18) (Variovorax sp. SCN45)
MPVPLNHSLPGERDAYLFHEGTHARLYDLLGCHPQPGGGARFAVWAPNAESVSVVGDWNY
WSGDADPLAPSPDHTGIWLGHVPNAGLGQAYKYRIRSRHGGFTVDKADPVAFSAEHPPAT
GSRICELAYEWGDDEWMATRGMHNSLEAPMSVYEVHLGSWRRHDGQFLGYREIAHELAAY
VKKMGFTHVELMPVTEHPFYGSWGYQTTGYFAPTARYGSPQDFMYLVDHLHQQGIGVLLD
WVPSHFPTDEHGLAFFDGTHLYEHADPRQGFHPEWNSSIFNYGRPEVRSFLVSSGLFWLD
LYHLDGLRVDAVASMLYLDYARKHGEWIPNRHGGRENLEAIDFLQTLNRAVYREFPDTLT
VAEESTAWPRVSRPTDMDGLGFGEKWNMGWMHDTLAYMKEDPVNRKYHHHKLTFSLVYAF
HENFVLPLSHDEVVHGKGSLINKMPGDSWQQFANLRALFGYMWAHPGKKLLFMGGEFGQR
REWTHDGELEWWVTQQQGHGGLQRLVAQLNRVYRESPALHQLDFSAAGFEWITADDAERS
VFAFLRKASDGSPPLLVVCNMTPVPRTNYLLGVPPGEGGGFWRELINTDAAEFGGAGWGN
LGGVEAAPVRSHGRMQSVCLTLPPLSTLILEQRPSP