Protein Info for GFF766 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Choline-sulfatase (EC 3.1.6.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 PF00884: Sulfatase" amino acids 4 to 335 (332 residues), 104 bits, see alignment E=1.5e-33 PF16347: SGSH_C" amino acids 283 to 357 (75 residues), 44.6 bits, see alignment E=2.5e-15

Best Hits

KEGG orthology group: None (inferred from 99% identity to stt:t2010)

Predicted SEED Role

"Choline-sulfatase (EC 3.1.6.6)" in subsystem Choline Transport or Choline and Betaine Uptake and Betaine Biosynthesis (EC 3.1.6.6)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.6.6

Use Curated BLAST to search for 3.1.6.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (495 amino acids)

>GFF766 Choline-sulfatase (EC 3.1.6.6) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MKAIILLFDSLNKNYLPPYGDLLTKAPNFQRLAAHAATFDNSYVGSMPCMPARRELHTGR
YNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDGGGNYHNRYSSWDVVRGQE
GDHWKASVGEPPIPEVLRVPQKQTGGGVSGLWRHDWANREYIQQEADFPQTKVFDAGCDF
IHKNHAEDNWLLQVETFDPHEPFYTTEEYLSLYDDEWQGPHYDWPRGKVSESEEAIAHIR
CRYRALVSMCDRNLGRILDLMDEHDLWRDTMLIVGTDHGFLLGEHGWWAKNQMPYYNEVA
NNPLFIWDPRSAVCGARRQSLVQMIDWAPTLLDYFQQPIPADMQGQPLAKIIASDEPVRE
GALFGVFSGHVNVTDGRYVYMRAAQPGREHDIANYTLMPIKMNARYDVDELGKLSLAPPF
NFTKGLQVLRIPAREKYKGVNSFGHLLFDLRDDPQQQHPIHDEAIEARMINLLIRLMKEN
DAPAEQYRRLGLDVV