Protein Info for GFF760 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Isoleucyl-tRNA synthetase (EC 6.1.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 953 TIGR00392: isoleucine--tRNA ligase" amino acids 19 to 870 (852 residues), 837.3 bits, see alignment E=7.7e-256 PF00133: tRNA-synt_1" amino acids 32 to 670 (639 residues), 441.9 bits, see alignment E=5.3e-136 PF09334: tRNA-synt_1g" amino acids 62 to 202 (141 residues), 44.2 bits, see alignment E=2.9e-15 PF08264: Anticodon_1" amino acids 716 to 868 (153 residues), 89.5 bits, see alignment E=5e-29 PF06827: zf-FPG_IleRS" amino acids 915 to 941 (27 residues), 33.5 bits, see alignment (E = 7.3e-12)

Best Hits

Swiss-Prot: 58% identical to SYI_BURM1: Isoleucine--tRNA ligase (ileS) from Burkholderia multivorans (strain ATCC 17616 / 249)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 79% identity to aaa:Acav_3320)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (953 amino acids)

>GFF760 Isoleucyl-tRNA synthetase (EC 6.1.1.5) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSDTPTDYRSTLNLPDTPFPMRGDLPKREPGWVNSWNQEGLYKRLRDAREGAALFVLHDG
PPYANGQIHMGHAVNKVLKDMIVKSRQLAGFDAQYIPGWDCHGLPIENAIEKKHGRNLSR
DDMQAKSRAYATEQIGLQREDFKRLGILGDWERPYRTMDPANEAGEIRAFKRVIERGFVY
RGLKPVYWCFDCGSSLAEFEIEYADKKSDTLDVAFEANDPAALAKAFGLAALPAGKTGFA
VIWTTTAWTIPANQALNAHPGLTYALVDTPRGVLLLAESLVEKCLERFQLEGQVIATAPG
EALRDLSFRHPLYDADAGYRRLSPLYLADYVSDSDGTGIVHSAPAYGLDDFNSCVSHGLA
YDDILNPVQGNGTYAADFPLFGGQHIWKAVPAILEALKNAGRLMATQPITHSYPHCWRHK
TPVIYRAAAQWFIRMDEGEGVFTKDKAPRTLRQTALDAIEHTHFFPENGKARLHDMIANR
PDWCISRQRSWGVPIPFFLHKDSGELHPRTMEILDQAADIVEKGGIEAWSRVTAEDILGA
QDAPNYTKSQDILEVWFDSGSTFQHVLRGSHAKAYVNGAFHSQGPEADLYLEGHDQHRGW
FHSSLLLGCALHDRAPYRGLLTHGFTVDSQGRKMSKSLGNGIDPQDVTKKLGAEIIRLWV
AASDYSGDIAGDDKILARVVDAYRRIRNTLRFLLANVSDFDPATDAVPLDQMLEIDRYAL
ARAAQFQAEVLAHYDVYEFHPVVAKLQVYCSEDLGAFYLDVLKDRLYTTAPKSLARRSAQ
TALHQITHAMLRWMAPFMSFTAEEAWPVLAGAQNQGSIFFETYAQLPNADEALLAKWTRL
CEVRNLVNKEIETVRTAGQVGASLQANVRLTAPADDHALLASLGDDLKFALIVSAVELLA
GDELAVQVVPSTAAKCERCWHYRDDVGTDAAHPTLCGRCTSNLFGAGEPRTVA