Protein Info for GFF76 in Xanthobacter sp. DMC5

Annotation: Holliday junction ATP-dependent DNA helicase RuvB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 343 PF05496: RuvB_N" amino acids 20 to 178 (159 residues), 265.4 bits, see alignment E=5.5e-83 TIGR00635: Holliday junction DNA helicase RuvB" amino acids 24 to 326 (303 residues), 451 bits, see alignment E=8.5e-140 PF00004: AAA" amino acids 55 to 177 (123 residues), 71.8 bits, see alignment E=2.7e-23 PF17864: AAA_lid_4" amino acids 181 to 254 (74 residues), 101.3 bits, see alignment E=7.2e-33 PF05491: RuvB_C" amino acids 256 to 325 (70 residues), 90.3 bits, see alignment E=2.1e-29

Best Hits

Swiss-Prot: 85% identical to RUVB_AZOC5: Holliday junction ATP-dependent DNA helicase RuvB (ruvB) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K03551, holliday junction DNA helicase RuvB (inferred from 95% identity to xau:Xaut_3069)

MetaCyc: 64% identical to Holliday junction branch migration complex subunit RuvB (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Holliday junction DNA helicase RuvB" in subsystem DNA-replication or RuvABC plus a hypothetical

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (343 amino acids)

>GFF76 Holliday junction ATP-dependent DNA helicase RuvB (Xanthobacter sp. DMC5)
MNTRLLSGEKRGDDAADSTLRPQLLSEFVGQAQARANLEIFIKAARARGEALDHVLFVGP
PGLGKTTLAQIMSREMGVGFRSTSGPVIAKAGDLAAILTNLEERDVLFIDEIHRLSPAVE
EILYPAMEDYELDLVIGEGPAARSVKISLPKFTLVGATTRSGLLTTPLRDRFGIPVRLQF
YTVEELEKIVVRGARVMGIAIAPDGATEIARRARGTPRIAGRLLRRVRDFALVAEAERID
RKIADRALLALEVDAAGLDAMDRRYLTVIAEFYGGGPVGVDTLAAALSEPRDAIEEIVEP
FLVQQGFIQRTPRGRMLTGAAFRHLGLAEPPSVKQMPLFDEGE