Protein Info for GFF746 in Xanthobacter sp. DMC5

Annotation: Methylenetetrahydrofolate--tRNA-(uracil-5-)-methy ltransferase TrmFO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 481 TIGR00137: tRNA:m(5)U-54 methyltransferase" amino acids 17 to 473 (457 residues), 536.8 bits, see alignment E=1.9e-165 PF01134: GIDA" amino acids 18 to 389 (372 residues), 332.5 bits, see alignment E=2e-103

Best Hits

Swiss-Prot: 90% identical to TRMFO_XANP2: Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO (trmFO) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K04094, glucose inhibited division protein Gid (inferred from 90% identity to xau:Xaut_4314)

Predicted SEED Role

"tRNA:m(5)U-54 MTase gid" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (481 amino acids)

>GFF746 Methylenetetrahydrofolate--tRNA-(uracil-5-)-methy ltransferase TrmFO (Xanthobacter sp. DMC5)
MPDTFWPDRPNGDNVAPVHVVGGGLAGSEASWQLATRGIPVVLHEMRPVRGTEAHLTDGL
AELVCSNSFRSDDPQGNAVGVLHAEMRRAGSLILKAADANQVPAGGALAVDREGFSRAVT
AALEGHPLIEIRREEITGLPPAEWDNVILATGPLTSPALAEAILGLTGESALAFFDAIAP
IVHFDSIDMGKAWFQSRYDKAGPGGTGADYINCPMDEAQYNAFVDALISGDKAPVRDFEA
KTPYFDGCLPIEVMAERGRETLRFGPMKPVGLTNPHAPDVKAYAIVQLRQDNKLGTLYNM
VGFQTKLRHGEQARVFRTIPGLENAEFARLGGLHRNTYLNSPRLLDGTLRLKADPRLRFA
GQITGCEGYVESAAVGLMAGRFAAAERLGLDVPLPPLTTAHGALLAHITGGHIEAEADGG
PRSFQPMNVNFGLFPPLAEAPKGEGGKRLRGAEKTLAKKKALAARALADILTWLDSPQQA
A