Protein Info for GFF7377 in Variovorax sp. SCN45

Annotation: Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01722: methylmalonate-semialdehyde dehydrogenase (acylating)
  • amino acids 14 to 490 (477 residues), 730.3 bits, E=4.7e-224
• PF00171: Aldedh
  • amino acids 24 to 486 (463 residues), 472.8 bits, E=5.1e-146

Best Hits

Swiss-Prot: 60% identical to MMSA_HUMAN: Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial (ALDH6A1) from Homo sapiens

KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 97% identity to vpe:Varpa_2387)

MetaCyc: 78% identical to methylmalonate-semialdehyde dehydrogenase subunit (Burkholderia cepacia R34)
Methylmalonate-semialdehyde dehydrogenase (acylating). [EC: 1.2.1.27]

Predicted SEED Role

"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)

MetaCyc Pathways

• β-alanine degradation II (2/2 steps found)
• β-alanine degradation I (1/2 steps found)
• propanoyl-CoA degradation II (3/5 steps found)
• L-valine degradation I (5/8 steps found)
• 2,4-dinitrotoluene degradation (2/7 steps found)
• myo-inositol degradation I (1/7 steps found)
• myo-, chiro- and scyllo-inositol degradation (1/10 steps found)

KEGG Metabolic Maps

• Inositol phosphate metabolism
• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.27

Use Curated BLAST to search for 1.2.1.27

Protein Sequence (507 amino acids)

>GFF7377 Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (Variovorax sp. SCN45)
MDATTTPSTQVATVKLLIGGKLVESKTTEWRDVVNPATQQVLARVPFATPAELDAAVASA
KEAFKTWRKTPIGTRARIFLKLQQLIRENMGELAAILTAEQGKTLPDAEGDVFRGLEVVE
HASNIGNLQLGELANNVANGVDTYTLLQPLGVCAGITPFNFPAMIPLWMFPMAIVTGNTF
VLKPSEQDPMVTMRLCELALEAGIPPGVLNVVHGGEAIVNGICDHKDIKAISFVGSTKVG
THVYNRATLAGKRAQCMMGAKNHAIVMPDANKEQTLNALAGASFGAAGQRCMAVSVAVLV
GEARNWVPELIEKAKTLKIGAGTEKGVDVGPLVSCAAYDRVNGLIERGEADGAKLVLDGR
KPTVPGYEKGNFVGPTIFTGVKPGMTIYEQEVFGPVLCIADAETIDEAIDLINSNPNGNG
TAIFTQSGAAARRFQEDIDVGQVGINVPIPVPVPMFSFTGSRASKLGDLGPYGKQVIMFY
TQTKTVTARWFDDSTVSHGVNTTISLK