Protein Info for GFF7304 in Variovorax sp. SCN45

Annotation: Limit dextrin alpha-1,6-maltotetraose-hydrolase (EC 3.2.1.196)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 TIGR02100: glycogen debranching enzyme GlgX" amino acids 5 to 673 (669 residues), 877.2 bits, see alignment E=3.4e-268 PF02922: CBM_48" amino acids 9 to 97 (89 residues), 61.2 bits, see alignment E=1.4e-20 PF00128: Alpha-amylase" amino acids 188 to 294 (107 residues), 26.3 bits, see alignment E=8.3e-10

Best Hits

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 80% identity to vpe:Varpa_0441)

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (700 amino acids)

>GFF7304 Limit dextrin alpha-1,6-maltotetraose-hydrolase (EC 3.2.1.196) (Variovorax sp. SCN45)
MLSPGKPFPLGATPMQQGVNFALAAPTAEAVDLCIFDTTGKNEQQRLRLPQQTDGIWHGH
LPSGKPGLVYGYRVHGPWSPKEGKRFNPAKLLLDPYAREIVGTYDGSDIFLGHDPANPDK
RDTRDNAAAALKARITAANERPSAGHARIPATDRVLYELHVKGQTKLHPAIPAPLRGSYA
GLAEPAMLDHLQRLGVTTLSLMPVQYRADEQRLLRMGLSNYWGYTTIGWFAPEPRYWSGR
PGTTPAGEFRAMADAIHARGMELVIDVVYNHSAETDEFGPTLSLRGIDNALYYRLRPDDR
ALYENWAGTGNCLDLTEPRVLRLVMDSLRFWACEMGVDGFRFDLAPVLGRDAGSNAFDPR
APFFAAIAQDPVLSRTLLIAEPWDLGPGGYRLGEFPPGWLEWNDRYRDTQRGFWLRQDQA
DKAGLGDFAHRFTASSTQFAHDGRAPTASVNFVTAHDGFTLRDLVSYNERHNLANGEDNR
DGHGHNMSNNCGVEGPSDDPAVLARRERLRRALLATLMLSQGTPMLLAGDELGHTQQGNN
NAYCQDNETTWLAWIGAASDPSSDAARMSAFVGRLAALRREAPALRSARWWPAEPPEGTP
PGIRWLRPNGEPMAPADWQTGTALAILFAGSDATDDAGERKQGAWLVMVNAGPDTVQFRL
PAGAWRLCLSSDPGHDPGNVTRPLDGTVPVPSSSLGIART