Protein Info for GFF7302 in Variovorax sp. SCN45

Annotation: Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 TIGR02095: glycogen/starch synthase, ADP-glucose type" amino acids 1 to 489 (489 residues), 523.5 bits, see alignment E=2.5e-161 PF08323: Glyco_transf_5" amino acids 2 to 247 (246 residues), 260 bits, see alignment E=3.7e-81 PF00534: Glycos_transf_1" amino acids 302 to 456 (155 residues), 34.9 bits, see alignment E=1.6e-12 PF13692: Glyco_trans_1_4" amino acids 307 to 451 (145 residues), 28.8 bits, see alignment E=2e-10

Best Hits

Swiss-Prot: 86% identical to GLGA_VARPS: Glycogen synthase (glgA) from Variovorax paradoxus (strain S110)

KEGG orthology group: K00703, starch synthase [EC: 2.4.1.21] (inferred from 88% identity to vpe:Varpa_0439)

Predicted SEED Role

"Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21)" in subsystem Glycogen metabolism (EC 2.4.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.21

Use Curated BLAST to search for 2.4.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (496 amino acids)

>GFF7302 Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21) (Variovorax sp. SCN45)
MRILQVSAELFPLLKTGGLADIAGALPLALMAEGQDARVLLPGFPAITRGVRELAPVAEF
DAPWGERFALRLGHVAVDGTPGIPAYVIDAPALYDRPGNPYEDNSRQPYGDNHRRFALLG
WAAARLAQGLDPSWQPEVVHAHDWHAALAPAYLHFAREAGQPGASRVGSVFTVHNLAYQG
LFAPWNFAELGLPGPAFQMNGVEYHGQVSFMKGGLCYADRLTTVSPTYALEIQTPEQGCG
LDGLLRQRSGVLSGILNAVDDQVWNPATDPALVQGYHTPEGRHMAGKARCKSVLQHQLGL
AERPDTPLFILVSRLTEQKGLGLVLDGLDALLAQGGQLALLGSGEGWLEDAFRQRAAAAP
QSVSVTIGYDETLAHQLFGAGDVTLVPSLFEPCGLTQMYGLKYGSLPLVRRVGGLADTVV
DSTLEDLAGGEATGFVFDRFEVADYQRAVRRAFALYQRAPDWRRVRGNAMRRPADWATAA
ARYIDVYRQALAAAAA