Protein Info for GFF7266 in Variovorax sp. SCN45

Annotation: L-threo-3-hydroxyaspartate ammonia-lyase (EC 4.3.1.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 55% identical to LTHAD_YEAST: L-threo-3-hydroxyaspartate ammonia-lyase (SRY1) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 62% identity to bpt:Bpet0902)

MetaCyc: 55% identical to L-threo-3-hydroxy aspartate ammonia-lyase (Pseudomonas sp. T62)
Threo-3-hydroxyaspartate ammonia-lyase. [EC: 4.3.1.16]

Predicted SEED Role

"Threonine dehydratase, catabolic (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis or Glycine and Serine Utilization or Threonine anaerobic catabolism gene cluster or Threonine degradation (EC 4.3.1.19)

MetaCyc Pathways

superpathway of branched chain amino acid biosynthesis (17/17 steps found)
superpathway of L-isoleucine biosynthesis I (13/13 steps found)
L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
superpathway of L-threonine metabolism (14/18 steps found)
L-methionine degradation II (3/3 steps found)
L-threonine degradation I (5/6 steps found)
L-threonine degradation V (1/2 steps found)
hypoglycin biosynthesis (4/14 steps found)
cyclosporin A biosynthesis (2/15 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.19

Use Curated BLAST to search for 4.3.1.16 or 4.3.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (331 amino acids)

>GFF7266 L-threo-3-hydroxyaspartate ammonia-lyase (EC 4.3.1.16) (Variovorax sp. SCN45)
LIAQDIGTPATAEDVLVAARALQGVAHRTPVMQSHTLNERLGAEIFFKCENLQRMGAFKF
RGAFNAVAQLDPERRAAGVITYSSGNHAQATALAARLLGTRATILMPGDTPTVKRDSTRG
YGAEVVLYDRLGDDREAMCATLVKARGLTLIHPFDDNRVIAGQGTAALELIQEVGELDSL
LTPLGGGGLLAGSALAAHLLSPRCQVVGVEPTASDDGQQSLRAGRIVRISPPQSIAEGAL
GTQLGERNFEIIRHHVHDVVTVEDASIVHAMQWLAQRMKMVVEPTGALGLGALLEHVLPV
HRKRVGVILSGGNVDLRRYAALLAGAPTAID