Protein Info for GFF7254 in Variovorax sp. SCN45

Annotation: Mannonate dehydratase (EC 4.2.1.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 318 PF03786: UxuA" amino acids 135 to 290 (156 residues), 100.6 bits, see alignment E=4.1e-33

Best Hits

KEGG orthology group: K01686, mannonate dehydratase [EC: 4.2.1.8] (inferred from 54% identity to azl:AZL_a04740)

Predicted SEED Role

"Mannonate dehydratase (EC 4.2.1.8)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 4.2.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (318 amino acids)

>GFF7254 Mannonate dehydratase (EC 4.2.1.8) (Variovorax sp. SCN45)
VRTCSTSSWLNPLRVLSLHKIRGDSLDVFALDVGSILLDSLTDLDGARKQRAMLAANIQK
AADAGITCLKYNVQMVGITRTGLKPGRGGAQNSGFVYAEYSADEDRKHSYWGVGYPSNQE
EGGSASAALCGQKMAREVPPVTEKAGWDAIEFLVEGLVPAADKAGVRLACHPHDPAYPVG
GLNGIEHVVGSADGIRRFLALSSSKNHGLNFCQGTVAEMFKEPGKHMIPVIEEFASTGRI
FMVHFRNIRGGYLDFQEVFPDEGDVDMYEAVKAYQRGGYAGPLCPDHVPVSEIDEGRERF
MSFALGYTRGLLQAAGVR