Protein Info for GFF725 in Variovorax sp. SCN45

Annotation: TonB-dependent siderophore receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 834 signal peptide" amino acids 1 to 43 (43 residues), see Phobius details PF07660: STN" amino acids 76 to 125 (50 residues), 32.2 bits, see alignment 1.1e-11 PF07715: Plug" amino acids 178 to 278 (101 residues), 62.4 bits, see alignment E=7.8e-21 TIGR01783: TonB-dependent siderophore receptor" amino acids 180 to 834 (655 residues), 386.8 bits, see alignment E=1.2e-119 PF00593: TonB_dep_Rec_b-barrel" amino acids 384 to 802 (419 residues), 200 bits, see alignment E=2e-62

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 67% identity to dac:Daci_3273)

Predicted SEED Role

"TonB-dependent siderophore receptor" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (834 amino acids)

>GFF725 TonB-dependent siderophore receptor (Variovorax sp. SCN45)
MALPRFRTSDNRRATPPLTAAAHGARCLCIALAMASGGIASAQQAAQPAAARPAHDIPAG
ALGPALSRYAAESGITLSFEPALAEGRSTAGLRGSYALSDGFARLLAGTGLEAVPRAGGG
YTLRQAAARAAPAAAADGDTTTLSEVRVTAQAERSAVTEGSGSYTSRVVTVGKGEQTLKE
IPQSVTVVTRQKMDDQNLTTIDSVLANATGITMYNSPMGGRYVYSRGFMADTYQFDGVNR
AFYYPQANNFTSNTAMLDRVEIVRGATGLLQGAGTPSATINMVRKRPLAEKQLQLSASAG
SWNNYRTEVDATGPLNEAGTLRGRAVASFDDRDYFYDVAKSRTGVLYGVLEYDIAPQTRL
TVGASQEKLRSVPFFHGLPRYNTGADIRLDRSTFLGANWNRWNSEQTSVFTELAHRFNDD
WTLKATATYTREKHHIKYMYSDGAINPATMAGSTTFSGLFDFETDNKGIDLSLDGKFDAF
GRRHSVSLGANTGRMANDGDFGLISLGVRNNVFNPNRLVAQPGTAEFLARRYRGAATITE
MKQSGAYGVARFSVSDPLTLVLGARVTNYDWRSRYRDTGEVYIDPYRSHGVVTPYGGLIY
ALNNQWSAYVSYADIFQPQNSVTASGAVLDPIKGKNYEAGLKGELMDGRLNASFAVFRID
QNNRAEQDMVNPCSVGSYCYISSGRVRSQGIDAEISGEIARGWQIFGGYTLNSFRYLNNT
SNAGVAFASTYTPKHILRLWTDYRLPGALNAWTVGGGVNFQTESSRTTGSIKVAQPSYAV
WTARVSYQIDRNWSLALNVSNLFDKTYYQTVGAPGWGNFYGEPRNTTLTLRGRF