Protein Info for GFF7230 in Variovorax sp. SCN45

Annotation: Threonine dehydrogenase and related Zn-dependent dehydrogenases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 345 PF08240: ADH_N" amino acids 25 to 128 (104 residues), 109 bits, see alignment E=1.1e-35 PF00107: ADH_zinc_N" amino acids 177 to 289 (113 residues), 72.8 bits, see alignment E=2.7e-24

Best Hits

KEGG orthology group: None (inferred from 84% identity to pzu:PHZ_p0136)

MetaCyc: 55% identical to NAD+-dependent secondary alcohol dehydrogenase II monomer (Gordonia sp. TY-5)
1.1.1.M22 [EC: 1.1.1.M22]

Predicted SEED Role

"Threonine dehydrogenase and related Zn-dependent dehydrogenases" in subsystem Threonine degradation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.M22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (345 amino acids)

>GFF7230 Threonine dehydrogenase and related Zn-dependent dehydrogenases (Variovorax sp. SCN45)
MKALVYQGPGLKSLEDRPKPEIQAPGDAIVRILKTTICGTDLHILKGDVPSCTPGRILGH
EGVGVVETVGNGVTAFHPGDHVLISCISSCGKCENCRRGMYSHCATGGWILGNTIDGTQA
EYVRIPHADTSLYPVPAGADEEALVMLSDILPTGFECGVLNGKVAPGSTVAIVGAGPIGL
ATLLTAQFYSPSQLILIDLDDGRLEVARRFGATHTINSADGNAARAVLALTGGRGVDVSI
EAVGIPATFVLCQDIVAPGGTIANIGVHGHAADLHLERLWSQNIAITTRLVDTVSTPMLL
KTVQSRKVDPARLITHRFRLDQVIDAYDTFARAAETHALKVLIEA